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Titolo:
UV-A irradiation induces a decrease in the mitochondrial respiratory activity of human NCTC 2544 keratinocytes
Autore:
Djavaheri-Mergny, M; Marsac, C; Maziere, C; Santus, R; Michel, L; Dubertret, L; Maziere, JC;
Indirizzi:
Hop St Louis, INSERM, U312, Dermatol Lab, F-75475 Paris, France Hop St Louis Paris France F-75475 2, Dermatol Lab, F-75475 Paris, France Fac Med Necker Enfants Malad, F-75730 Paris 15, France Fac Med Necker Enfants Malad Paris France 15 d, F-75730 Paris 15, France Hop Nord Amiens, CHU Amiens, Biochim Lab, F-80054 Amiens 01, France Hop Nord Amiens Amiens France 01 Biochim Lab, F-80054 Amiens 01, France Museum Natl Hist Nat, INSERM, U312, Lab Photobiol, F-75231 Paris 05, France Museum Natl Hist Nat Paris France 05 Photobiol, F-75231 Paris 05, France
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 6, volume: 34, anno: 2001,
pagine: 583 - 594
SICI:
1071-5762(2001)34:6<583:UIIADI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPORPHYRIN DERIVATIVE PHOTOFRIN; CULTURED HUMAN FIBROBLASTS; INDUCED LIPID-PEROXIDATION; SINGLET OXYGEN; CYTOCHROME-C; INDUCED EXPRESSION; ULTRAVIOLET; ACTIVATION; APOPTOSIS; INHIBITION;
Keywords:
UV-A; mitochondria; intracellular ATP; oxygen consumption; MnSOD; mitochondrial membrane potential (Delta Psi);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Djavaheri-Mergny, M Inst Curie, Rech Sect Biol, INSERM, U365, 26 Rue Ulm, F-75231 Paris 05, France Inst Curie 26 Rue Ulm Paris France 05 aris 05, France
Citazione:
M. Djavaheri-Mergny et al., "UV-A irradiation induces a decrease in the mitochondrial respiratory activity of human NCTC 2544 keratinocytes", FREE RAD RE, 34(6), 2001, pp. 583-594

Abstract

UV-A irradiation caused a dose-dependent decrease in cellular oxygen consumption (56%) and ATP content (65%) in human NCTC 2544 keratinocytes, one hour after treatment. This effect was partially reversed by maintaining the irradiated cells in normal culture conditions for 24 h. Using malate/glutamate or succinate as substrates for mitochondrial electron transport, the oxygen uptake of digitonin-permeabilised cells was greatly inhibited followingUV-A exposure. These results strongly suggest that UV-A irradiation affects the state 3 respiration of the mitochondria. However, under identical conditions, UV-A exposure did not reduce the mitochondrial transmembrane potential. The antioxidant, vitamin E inhibited UV-A-induced lipid peroxidation,but did not significantly prevent the UV-A-mediated changes in cellular respiration nor the decrease in ATP content, suggesting that these effects were not the result of UV-A dependent lipid peroxidation. UV-A irradiation also led to an increase in MnSOD gene expression 24 hours after treatment, indicating that the mitochondrial protection system was enhanced in response to UV-A treatment. These findings provide evidence that impairment of mitochondrial respiratory activity is one of the early results of UV-A irradiation for light doses much lower than the minimal erythemal dose.

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Documento generato il 30/09/20 alle ore 09:25:40