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Titolo:
Arsenite induces oxidative DNA adducts and DNA-protein cross-links in mammalian cells
Autore:
Wang, TS; Hsu, TY; Chung, CH; Wang, ASS; Bau, DT; Jan, KY;
Indirizzi:
Acad Sinica, Inst Zool, Taipei 11529, Taiwan Acad Sinica Taipei Taiwan 11529 Sinica, Inst Zool, Taipei 11529, Taiwan Chung Shan Med & Dent Coll, Dept Life Sci, Taichung, Taiwan Chung Shan Med& Dent Coll Taichung Taiwan t Life Sci, Taichung, Taiwan Natl Def Med Ctr, Grad Inst Life Sci, Taipei, Taiwan Natl Def Med Ctr Taipei Taiwan Ctr, Grad Inst Life Sci, Taipei, Taiwan
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 3, volume: 31, anno: 2001,
pagine: 321 - 330
SICI:
0891-5849(20010801)31:3<321:AIODAA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER OVARY CELLS; ULTRAVIOLET-LIGHT; REACTIVE OXYGEN; SODIUM ARSENITE; DRINKING-WATER; NITRIC-OXIDE; STRAND BREAKS; COMET ASSAY; NB4 CELLS; BLADDER;
Keywords:
comet assay; arsenic; DNA damages; DNA-protein cross-links; 8OH-dG; peroxynitrite; hypochlorous acid; hydroxyl radicals; free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Jan, KY Acad Sinica, Inst Zool, Taipei 11529, Taiwan Acad Sinica Taipei Taiwan 11529 Inst Zool, Taipei 11529, Taiwan
Citazione:
T.S. Wang et al., "Arsenite induces oxidative DNA adducts and DNA-protein cross-links in mammalian cells", FREE RAD B, 31(3), 2001, pp. 321-330

Abstract

Arsenic is generally recognized as a nonmutagenic carcinogen because sodium arsenite induces DNA damage only at very high concentrations. In this study we demonstrate that arsenite concentrations above 0.25 muM induce DNA strand breaks in both human leukemia cells and Chinese hamster ovary cells. Therefore, DNA damage may be involved in arsenic-induced carcinogenesis. Formamidopyrimidine-DNA glycosylase and proteinase K greatly increased DNA strand breaks in arsenite-treated cells, providing evidence that a large portion of arsenite-induced DNA strand breaks come from excision of oxidative DNA adducts and DNA-protein cross-links. Because DNA strand breaks appear only temporarily during excision repair, the level of detectable DNA strand breaks will be low at any given time point. For this reason many previous studies have only detected low levels of DNA strand breaks. We also show that catalase, and inhibitors of calcium, nitric oxide synthase, superoxide dismutase, and myeloperoxidase, could modulate arsenite-induced DNA damage. We conclude that arsenite induces DNA adducts through calcium-mediated production of peroxynitrite, hypochlorous acid, and hydroxyl radicals. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:19:56