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Titolo:
Nonanaphylactic synthetic peptides derived from B cell epitopes of the major grass pollen allergen, Phl p 1, for allergy vaccination
Autore:
Focke, M; Mahler, V; Ball, T; Sperr, WR; Majlesi, Y; Valent, P; Kraft, D; Valenta, R;
Indirizzi:
Univ Vienna, Vienna Gen Hosp, AKH, Dept Pathophysiol,Mol Immunopathol Grp,A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 mmunopathol Grp,A-1090 Vienna, Austria Univ Vienna, Vienna Gen Hosp, AKH, Dept Hematol & Hemostaseol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 & Hemostaseol, A-1090 Vienna, Austria Univ Erlangen Nurnberg, Dept Dermatol, D-8520 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-8520 D-8520 Erlangen, Germany
Titolo Testata:
FASEB JOURNAL
fascicolo: 9, volume: 15, anno: 2001,
pagine: NIL_120 - NIL_145
SICI:
0892-6638(200107)15:9<NIL_120:NSPDFB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-EPSILON-RI; IGG MONOCLONAL-ANTIBODIES; ANTIGEN-PRESENTING CELLS; RECOMBINANT ALLERGENS; IMMUNOTHERAPY; BINDING; DISEASES; BIRCH; DIAGNOSIS; BET-V-1;
Keywords:
Type I allergy; IgE; non-allergenic allergy vaccine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Valenta, R Univ Vienna, Vienna Gen Hosp, AKH, Dept Pathophysiol,Mol Immunopathol Grp,Waehringer Guertel 18-20, A-1090 Vienna, Austria Univ Vienna Waehringer Guertel 18-20 Vienna Austria A-1090 ria
Citazione:
M. Focke et al., "Nonanaphylactic synthetic peptides derived from B cell epitopes of the major grass pollen allergen, Phl p 1, for allergy vaccination", FASEB J, 15(9), 2001, pp. NIL_120-NIL_145

Abstract

Worldwide more than 200 million individuals are allergic to group 1 grass pollen allergens. We have used the major timothy grass pollen allergen Phl p 1, which cross-reacts with most grass-, corn-, and monocot-derived group 1 allergens to develop a generally applicable strategy for the production of hypoallergenic allergy vaccines. On the basis of the experimentally determined B cell epitopes of Phl p 1, we have synthesized five synthetic peptides. These peptides are derived from the major Phl p 1 IgE epitopes and werebetween 28-32 amino acids long. We demonstrate by nuclear magnetic resonance that the peptides exhibit no secondary and tertiary structure and accordingly failed to bind IgE antibodies from grass pollen allergic patients. The five peptides, as well as an equimolar mixture thereof, lacked allergenicactivity as demonstrated by basophil histamine release and skin test experiments in grass pollen allergic patients. When used as immunogens in mice and rabbits, the peptides induced protective IgG antibodies, which recognized the complete Phl p 1 wild-type allergen and group 1 allergens from other grass species. Moreover, peptide-induced antibodies inhibited the binding of grass pollen allergic patients IgE antibodies to the wild-type allergen. We thus demonstrate that synthetic hypoallergenic peptides derived from B cell epitopes of major allergens represent safe vaccine candidates for the treatment of IgE-mediated allergies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:51:26