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Titolo:
Investigations on the activity of bovine placental oestrogen sulfotransferase and -sulfatase from midgestation to parturition
Autore:
Hoffmann, B; Falter, K; Vielemeier, A; Failing, K; Schuler, G;
Indirizzi:
Univ Giessen, Gynakol & Androl Gross & Kleintiere Tierarztl, Klinik Geburtshilfe, D-35392 Giessen, Germany Univ Giessen Giessen Germany D-35392 urtshilfe, D-35392 Giessen, Germany Univ Giessen, Arbeitsgrp Biomath & Datenverarbeitung, Inst Vet Physiol, D-35392 Giessen, Germany Univ Giessen Giessen Germany D-35392 t Physiol, D-35392 Giessen, Germany
Titolo Testata:
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES
fascicolo: 5, volume: 109, anno: 2001,
pagine: 294 - 301
SICI:
0947-7349(2001)109:5<294:IOTAOB>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEROID-SULFATASE; ESTRONE SULFATASE; IMMUNOCYTOCHEMISTRY; EXPRESSION; RECEPTOR; GENES; LIVER; COW; RAT;
Keywords:
cattle; placenta; oestrogens; oestrogen sulfotransferase; oestrogen sulfatase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffmann, B Univ Giessen, Gynakol & Androl Gross & Kleintiere Tierarztl, Klinik Geburtshilfe, Frankfurter Str 106, D-35392 Giessen, Germany Univ Giessen Frankfurter Str 106 Giessen Germany D-35392 many
Citazione:
B. Hoffmann et al., "Investigations on the activity of bovine placental oestrogen sulfotransferase and -sulfatase from midgestation to parturition", EXP CL E D, 109(5), 2001, pp. 294-301

Abstract

To gain further information on the biological role of the high amounts of conjugated estrone (EIS) secreted by the bovine placenta, an in vitro assaysystem was developed to measure oestrogen sulfotransferase (OST) and oestrogen sulfatase (OS) activities in caruncular and cotyledonary homogenates and the respective subcellular fractions. Placental tissue was obtained from150 (n=3), 220 (n=4), 240 (n=3) and 270 days (n=3) pregnant and parturientcows (n=4). H-3-E1 and H-3-E1S served as substrates and 4-nitrophenyl sulfate potassium salt was used as a competitive inhibitor to block OS when testing for OST activity. OST-activity did not change during pregnancy and parturition and was higher (p <0.001) in cotyledonary than in caruncular tissue with mean conversions (median) of 61.8% and 41.6% after 30 min of homogenate incubation. On a subcellular level OST-activity was clearly associated with the cytosol. OS-activity was higher (p <0.001) in caruncular than in cotyledonary homogenates; it was constant during pregnancy (median of conversion: 88.0% and 66.4%, resp.), but was significantly decreased (p <0.05) atparturition (median of conversion: 48.1% and 30.6%, resp.). On a subcellular level in both the cotyledon and the caruncle highest OS-activities were detected in the microsomal and the mitochondrial fractions. The decreased placentomal OS-activities in parturient cows are inconsistent with a substantial role of OS in the prepartal increase of free oestrogens. These resultsalso suggest that bovine placental oestrogens may largely exert their action locally within the placentomes during most time of gestation as the enzymes catalyzing sulfoconjugation (i.e. inactivation of free estrogens) and desulfation (i.e. activation of conjugated oestrogens) are expressed in close proximity to each other. In this respect the finding of oestrogen receptors in caruncular stromal cells is of particular interest.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:24:37