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Titolo:
Activation of natural killer cells by bacillus Calmette-Guerin
Autore:
Brandau, S; Bohle, A;
Indirizzi:
Res Ctr Borstel, Div Immunotherapy, D-23845 Borstel, Germany Res Ctr Borstel Borstel Germany D-23845 herapy, D-23845 Borstel, Germany Med Univ Lubeck, Dept Urol, Lubeck, Germany Med Univ Lubeck Lubeck Germany Univ Lubeck, Dept Urol, Lubeck, Germany
Titolo Testata:
EUROPEAN UROLOGY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 518 - 524
SICI:
0302-2838(200105)39:5<518:AONKCB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERFICIAL BLADDER-CANCER; BLOOD MONONUCLEAR-CELLS; TUMOR-NECROSIS-FACTOR; INTRAVESICAL IMMUNOTHERAPY; BCG IMMUNOTHERAPY; IMMUNE-RESPONSE; INDUCTION; INTERLEUKIN-2; CARCINOMA; SUBSETS;
Keywords:
bacillus Calmette-Guerin; natural killer cells; bladder tumor; immunotherapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Brandau, S Res Ctr Borstel, Div Immunotherapy, Pk Allee 26B, D-23845 Borstel, Germany Res Ctr Borstel Pk Allee 26B Borstel Germany D-23845 , Germany
Citazione:
S. Brandau e A. Bohle, "Activation of natural killer cells by bacillus Calmette-Guerin", EUR UROL, 39(5), 2001, pp. 518-524

Abstract

Objectives: Using a human in vitro model we have previously identified so-called bacillus Calmette-Guerin (BCG)-activated killer (BAK) cells as potential effector cells in BCG immunotherapy. This study was designed to prove the hypothesis that BAK cells are a subpopulation of natural killer (NK) cells and to analyze the role of NK cells during BCG immunotherapy in vivo. Methods:After stimulation of mononuclear cells (MNCs) with BCG for 7 days CD3+ and CD56+ lymphocytes were depleted by magnetic cell separation. Subsequently, the cytotoxicity of the marker-negative cell population was testedin a radioactive release assay. Coexpression of CD56/CD16 and CD56/perforin was assessed by flow cytometry. The importance of NK cells for effective BCG immunotherapy in vivo was analyzed by comparing BCG treatment of bladder tumors bearing 'wild-type' C57BL/6 and NK-deficient beige mice,Results: BAK cells were shown to have a CD3-/CD56+ NK cell phenotype. Theyexpressed high amounts of perforin and low amounts of CD16, both of which are characteristic features of (activated) NK cells. BCG immunotherapy significantly prolonged survival in tumor-bearing C57BL/6 mice but was ineffective in NK-deficient beige mice. However, BCG treatment did not influence the frequency of pulmonary metastases in both mouse strains. Conclusions: Our data clearly indicate that stimulation of human MNCs withBCG leads to the activation of cytotoxic lymphocytes with NK cell phenotype. These killer cells express perforin and CD16, two molecules involved in NK cell cytotoxicity. Finally, ineffective BCG treatment of beige mice suggests a key role for NK cells during BCG immunotherapy in vivo. Copyright (C) 2001 S. Karger AG, Basel.

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Documento generato il 16/07/20 alle ore 19:01:34