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Titolo:
Fluticasone and salmeterol downregulate in vitro, fibroblast proliferationand ICAM-1 or H-CAM expression
Autore:
Silvestri, M; Fregonese, L; Sabatini, F; Dasic, G; Rossi, GA;
Indirizzi:
G Gaslini Inst Children, Dept Pulm Dis, Div Pulm, I-16147 Genoa, Italy G Gaslini Inst Children Genoa Italy I-16147 v Pulm, I-16147 Genoa, Italy Glaxo Wellcome SpA, Verona, Italy Glaxo Wellcome SpA Verona ItalyGlaxo Wellcome SpA, Verona, Italy
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 1, volume: 18, anno: 2001,
pagine: 139 - 145
SICI:
0903-1936(200107)18:1<139:FASDIV>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LUNG FIBROBLASTS; INTERCELLULAR-ADHESION MOLECULE-1; BRONCHIAL EPITHELIAL-CELLS; SUBEPITHELIAL FIBROSIS; AIRWAY INFLAMMATION; VCAM-1 EXPRESSION; GENE-EXPRESSION; GM-CSF; ASTHMA; CD44;
Keywords:
airway remodelling; beta(2)-agonists; bronchial asthma; corticosteroids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Rossi, GA G Gaslini Inst Children, Dept Pulm Dis, Div Pulm, Largo G Gaslini 5, I-16147 Genoa, Italy G Gaslini Inst Children Largo G Gaslini 5 Genoa Italy I-16147 y
Citazione:
M. Silvestri et al., "Fluticasone and salmeterol downregulate in vitro, fibroblast proliferationand ICAM-1 or H-CAM expression", EUR RESP J, 18(1), 2001, pp. 139-145

Abstract

beta (2)-adrenoreceptor agonists have pharmacological properties that may suggest an inhibitory effect on various aspects of the inflammatory and repair processes that characterize asthma. Since fibroblasts express beta (2)-adrenoreceptors, the effects of different concentrations (0.1 - 100 nM) of fluticasone propionate (FP), salmeterol(S) and their combination (FP + S) on lung fibroblast proliferation and adhesion molecule expression were evaluated. Stimulation of human foetal lung fibroblasts with a fibrogenic cytokine, basic fibroblast growth factor (bFGF), resulted in a [methyl-H-3] thymidine ([H-3]TdR) uptake, four-fold higher than that of control cultures (p = 0.0001) and was significantly inhibited by S, at all the concentrations tested (0.1 - 100 nM; p < 0.05). No changes in bFGF-induced cell proliferation were observed in the presence of FP (0.1 - 100 nM; p > 0.05, all comparisons). In addition, the association FP + S did not improve the inhibitory activity of S alone (p > 0.05. each comparison). An upregulation of intercellular adhesion molecule-1 (ICAM-1) expression was induced by tumour necrosis factor-alpha (TNF-alpha) (p = 0.0004), but not by interleukin-4 (IL-4) (p > 0.05), while none of the two cytokines were able to increase hyaluronic-cellular adhesion molecule (H-CAM) expression by lung fibroblasts (p > 0.05). A significant downregulation of ICAM-1 or H-CAM expression was demonstrated inthe presence of FP or S, at all concentrations, tested (0.1 - 100 nM and 100 nM; p < 0.01,each comparison). Interestingly, S (10 nM and 100 nM) was able to enhance the inhibitory activity of FP on ICAM-1 expression (p < 0.01), but not on H-CAM expression (p > 0.1). These results show that in human foetal lung fibroblasts, fluticasone propionate and salmeterol are effective in modulating in vitro, different lung fibroblast biological functions that are likely to be involved in airway remodelling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:24:37