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Titolo:
Surfactant proteins D and A in sputum
Autore:
Griese, M; Maderlechner, N; Bufler, P;
Indirizzi:
Univ Munich, Dr von Hauner Childrens Hosp, Kinderpoliklin, D-80336 Munich,Germany Univ Munich Munich Germany D-80336 inderpoliklin, D-80336 Munich,Germany
Titolo Testata:
EUROPEAN JOURNAL OF MEDICAL RESEARCH
fascicolo: 1, volume: 6, anno: 2001,
pagine: 33 - 38
SICI:
0949-2321(20010129)6:1<33:SPDAAI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS-SYNDROME; PULMONARY SURFACTANT; CYSTIC-FIBROSIS; ENDOTOXIN;
Keywords:
healthy subjects; induced sputum; cystic fibrosis; host defense; innate immunity; lungs; surfactant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Griese, M Univ Munich, Dr von Hauner Childrens Hosp, Kinderpoliklin, Pettenkoferstr 8A, D-80336 Munich, Germany Univ Munich Pettenkoferstr 8A MunichGermany D-80336 h, Germany
Citazione:
M. Griese et al., "Surfactant proteins D and A in sputum", EUR J MED R, 6(1), 2001, pp. 33-38

Abstract

The surfactant proteins SP-D and SP-A are collectins and components of theinnate immune system of the peripheral lungs. They bind to carbohydrates on microorganisms and promote their removal. In the larger airways their role and concentrations are not yet known. Sputum may thus potentially be useful to characterize the collectins in this compartment. Induced sputum was obtained from healthy adults and spontaneous sputum from patients with cystic fibrosis. ELISA and carbohydrate binding assays wereused to quantify the amount and functional capacity of the collectins in plugs or sol-fractions, prepared by centrifugation of the plugs. SP-D and SP-A were detectable in only about 50% of the sputum plugs. Varying the salt, detergent and calcium conditions of the assay did not improve the results. In samples with reproducibly detectable SP-D or SP-A, their carbohydrate binding capacity was zero. Sputum sol fraction and LPS inhibitedthe binding of the collectins to carbohydrate in the presence of calcium. Whereas SP-D and SP-A are abundant in the peripheral lung, their presence in sputum derived from the larger air-ways is variable and their carbohydrate binding capacity is lost.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:15:25