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Titolo:
Prostaglandin E-2 activates the ciliary beat frequency of cultured human nasal mucosa via the second messenger cyclic adenosine monophosphate
Autore:
Haxel, BR; Schafer, D; Klimek, L; Mann, WJ;
Indirizzi:
Univ Mainz, Dept Otorhinolaryngol, D-55101 Mainz, Germany Univ Mainz Mainz Germany D-55101 torhinolaryngol, D-55101 Mainz, Germany
Titolo Testata:
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
fascicolo: 5, volume: 258, anno: 2001,
pagine: 230 - 235
SICI:
0937-4477(200107)258:5<230:PEATCB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID METABOLITES; EPITHELIAL-CELLS; IN-VITRO; RESPIRATORY EPITHELIUM; CHLORIDE SECRETION; ADENYLATE-CYCLASE; MODULATION; FORSKOLIN; SINUSITIS; MEDIATORS;
Keywords:
ciliary beat frequency; cyclic AMP; prostaglandin E-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Haxel, BR Univ Mainz, Dept Otorhinolaryngol, Langenbechstr 1, D-55101 Mainz, Germany Univ Mainz Langenbechstr 1 Mainz Germany D-55101 Mainz, Germany
Citazione:
B.R. Haxel et al., "Prostaglandin E-2 activates the ciliary beat frequency of cultured human nasal mucosa via the second messenger cyclic adenosine monophosphate", EUR ARCH OT, 258(5), 2001, pp. 230-235

Abstract

Prostaglandins influence the ciliary beat frequency (CBF) of ciliated nasal epithelial cells and a stimulatory effect has been described for prostaglandin E-2? (PGE(2)). Until now, it is not known whether PGE(2) has direct ciliostimulatory properties or acts through a second messenger. In this study we investigated whether cyclic adenosine monophosphate (cAMP) is implicated in the signal transduction pathway of PGE(2)-induced activation of CBF. Ciliated cells of the nasal mucosa were cultured for up to 5 days whereafter the culture medium was removed and the cells were incubated with different concentrations of test solutions. The ciliated cells were recorded under a phase-contrast microscope and viewed in slow motion to count the frequency. PGE(2) led to a dose-dependent increase in CBF. This became significant at concentrations of 10(-10) and 10(-5) M (P < 0.01) but not at 10(-13) M (P > 0.05). Addition of cAMP (10(-10) to 10(-5) M) caused a significant (P <0.01) increase in CBF, whereas depletion of endogenous cAMP after pre-incubation with the adenylate cyclase activator forskolin (10(-5) M) prevented the PGE(2)-induced increase in CBF (P > 0.05). The ciliostimulatory effect of PGE(2) depends on an intact functioning of adenylate cyclase. These results point out that cAMP is directly implicated in the signal transduction pathway of PGE(2)-induced stimulation of CBF in cultured human ciliated cells of the nasal mucosa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:39:50