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Titolo:
Repeated low-dose treatment of rats with pilocarpine: low mortality but high proportion of rats developing epilepsy
Autore:
Glien, M; Brandt, C; Potschka, H; Voigt, H; Ebert, U; Loscher, W;
Indirizzi:
Sch Vet Med, Dept Pharmacol Toxicol & Pharm, D-30559 Hannover, Germany SchVet Med Hannover Germany D-30559 & Pharm, D-30559 Hannover, Germany
Titolo Testata:
EPILEPSY RESEARCH
fascicolo: 2, volume: 46, anno: 2001,
pagine: 111 - 119
SICI:
0920-1211(200108)46:2<111:RLTORW>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS RECURRENT SEIZURES; INDUCED STATUS EPILEPTICUS; TEMPORAL-LOBE EPILEPSY; BRAIN-DAMAGE; MODEL; LITHIUM; EPILEPTOGENESIS; STIMULATION; KAINATE;
Keywords:
seizures; lithium; epileptogenesis; status epilepticus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Loscher, W Sch Vet Med, Dept Pharmacol Toxicol & Pharm, Bunteweg 17, D-30559 Hannover, Germany Sch Vet Med Bunteweg 17 Hannover Germany D-30559 over, Germany
Citazione:
M. Glien et al., "Repeated low-dose treatment of rats with pilocarpine: low mortality but high proportion of rats developing epilepsy", EPILEPSY R, 46(2), 2001, pp. 111-119

Abstract

Systemic administration of pilocarpine in rats can result in a chronic behavioral state that is similar to human temporal lobe epilepsy. The pilocarpine model of epilepsy is widely used for studying the factors that contribute to the development of epilepsy as a consequence of status epilepticus (SE). For this purpose, pilocarpine is either administered alone at a high systemic dose or in combination with lithium, which markedly potentiates the convulsant effect of pilocarpine. Both experimental protocols, however, areassociated with high mortality rates. In the present study, we evaluated whether mortality rate in rats can be decreased by repeated administration of low doses of pilocarpine. The time the rats spent in SE was limited by diazepam. Preliminary experiments in lithium-free rats indicated that repeated low-dose administration of pilocarpine is too time-consuming to produce SE compared to single high-dose administration. All subsequent experiments were performed in lithium-pretreated rats. Single-dose injection of 30 mg/kgpilocarpine produced SE in approximately 70% of the animals, but 45% of the rats died although SE was interrupted by diazepam after 90 min. Repeated i.p. administration of 10 mg/kg pilocarpine at 30-min intervals resulted inSE after 2-4 injections, the mean dose of pilocarpine needed to induce SE was 26 mg/kg. When SE was interrupted after 90 min, mortality rate was below 10%, which was significantly lower compared to the protocol with one single administration of 30 mg/kg pilocarpine. In contrast to mortality rate, the development of spontaneous recurrent seizures did not differ between experimental protocols. Almost all rats which had experienced a SE of at least60 min developed chronic epilepsy. Average latency to the first spontaneous seizure was approximately 40 days. The frequency and severity of spontaneous seizures was not significantly different between protocols, although animal groups with repeated low-dose treatment tended to have higher frequencies of spontaneous seizures compared to single-dose administration. The present study demonstrates that systemic treatment of lithium-pretreated rats with several low doses of pilocarpine efficiently produces SE and chronic epilepsy with much lower mortality rates than single-dose pilocarpine. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:47:42