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Titolo:
A single (6-4) photoproduct inhibits plasmid DNA replication in xeroderma pigmentosum variant cell extracts
Autore:
Yao, JQ; Dixon, K; Carty, MP;
Indirizzi:
Univ Cincinnati, Med Ctr, Dept Environm Hlth, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 nm Hlth, Cincinnati, OH 45267 USA
Titolo Testata:
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
fascicolo: 1, volume: 38, anno: 2001,
pagine: 19 - 29
SICI:
0893-6692(2001)38:1<19:AS(PIP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMINE-THYMINE DIMER; ESCHERICHIA-COLI DINB; SIMIAN VIRUS-40 DNA; UV-INDUCED LESION; ULTRAVIOLET-LIGHT; POLYMERASE-ETA; POSTREPLICATION REPAIR; TRANSLESION SYNTHESIS; PYRIMIDINE DIMERS; EXCISION-REPAIR;
Keywords:
XP variant; (6-4) photoproduct; DNA polymerase eta; DNA replication; DNA damage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Carty, MP Natl Univ Ireland Univ Coll Galway, Dept Biochem, Galway, Ireland Natl Univ Ireland Univ Coll Galway Galway Ireland ay, Ireland
Citazione:
J.Q. Yao et al., "A single (6-4) photoproduct inhibits plasmid DNA replication in xeroderma pigmentosum variant cell extracts", ENV MOL MUT, 38(1), 2001, pp. 19-29

Abstract

The human skin cancer-prone disease xeroderma pigmentosum variant (XPV) results from a mutation in the human RAD30 gene, which encodes the lesion bypass DNA polymerase eta. XPV cells are characterized by delayed completion of DNA replication and increased mutagenesis following UV-irradiation. Usingextracts of an XPV lymphoblast cell line (GM2449C) that has a truncating mutation in the RAD30 gene, we investigated the effect of a (6-4) photoproduct and a cyclobutane pyrimidine dimer (CPD), at a unique -TT- site on either the leading or logging strand, on plasmid DNA replication. Compared to normal cell extracts, XPV cell extracts have a reduced capacity to carry out complete replication of DNA containing either a (6-4) photoproduct or a CPDon the leading strand, whereas there is little difference between the two cell extracts in replication of DNA containing a lesion on the logging strand. Inhibition of replication in the presence of a (6-4) photoproduct is attributed to arrest of nascent DNA strand synthesis at the lesion site; in XPV cell extracts, the proportion of arrested products is increased comparedto that of normal cell extracts. These results are consistent with a requirement for functional DNA polymerase eta in the replication of a double-stranded plasmid containing either a (6-4) photoproduct or a CPD, on the leading but not the lagging strand. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 09/04/20 alle ore 07:37:18