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Titolo:
RIP 140 modulates transcription of the steroidogenic acute regulatory protein gene through interactions with both SF-1 and DAX-1
Autore:
Sugawara, T; Abe, S; Sakuragi, N; Fujimoto, Y; Nomura, E; Fujieda, K; Saito, M; Fujimoto, S;
Indirizzi:
Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Hokkaido Univ, Sch Med, Dept Pediat, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Hokkaido Univ, Sch Med, Dept Obstet & Gynecol, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Asahikawa Med Coll, Dept Pediat, Asahikawa, Hokkaido 0788510, Japan Asahikawa Med Coll Asahikawa Hokkaido Japan 0788510 kkaido 0788510, Japan Natl Canc Ctr, Res Inst, Div Virol, Tokyo 1048638, Japan Natl Canc Ctr Tokyo Japan 1048638 Inst, Div Virol, Tokyo 1048638, Japan
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 8, volume: 142, anno: 2001,
pagine: 3570 - 3577
SICI:
0013-7227(200108)142:8<3570:R1MTOT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL HYPOPLASIA CONGENITA; PROMOTER ACTIVITY; FACTOR-I; DEVELOPMENTAL REGULATION; ENDOCRINE DEVELOPMENT; HORMONE-RECEPTOR; STAR GENE; EXPRESSION; CELLS; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, T Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Kita 15,Nishi 7, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Kita 15,Nishi 7 Sapporo Hokkaido Japan 0608638 n
Citazione:
T. Sugawara et al., "RIP 140 modulates transcription of the steroidogenic acute regulatory protein gene through interactions with both SF-1 and DAX-1", ENDOCRINOL, 142(8), 2001, pp. 3570-3577

Abstract

Coregulators have been suggested to act as a bridging apparatus between nuclear receptors and the transcriptional machinery. The orphan receptor SF-1plays a role in controlling the basal and cAMP-stimulated expression of the human steroidogenic acute regulatory protein gene. DAX-1 is the gene responsible for X-linked adrenal hypoplasia congenita and blocks steroid biosynthesis by impairing the expression of steroidogenic acute regulatory protein. In the present study we examined the role of coregulators in the actionsof SF-1 and DAX-1 on the human steroidogenic acute regulatory protein promoter. We found that the coregulator RIP 140 interacts with SF-1 in the yeast two-hybrid system. Glutathione-S-transferase pull-down assays and coimmunoprecipitations confirmed the interaction between RIP 140 and SF-1. RIP 140was also shown to interact with DAX-1. When an RIP 140 expression vector was introduced into Y-1 cells, basal and cAMP-stimulated human steroidogenicacute regulatory protein promoter activities decreased. The inhibitory effect of RIP 140 on human steroidogenic acute regulatory protein promoter activity was dependent upon the presence of SF-1. The cAMP response of an SF-1response element was inhibited by both RIP 140 and DAX-1 expression vectors at low concentrations of plasmids. We conclude that RIP 140 binds to the orphan nuclear receptor SF-1 and DAX-1 and modulates their actions on the human steroidogenic acute regulatory protein promoter.

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Documento generato il 23/09/20 alle ore 16:04:24