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Titolo:
Induction, distribution and modulation of upper airway allergic inflammation in mice
Autore:
Hussain, I; Randolph, D; Brody, SL; Song, SK; Hsu, A; Kahn, AM; Chaplin, DD; Hamilos, DL;
Indirizzi:
Washington Univ, Sch Med, Div Allergy & Immunol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 y & Immunol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Internal Med, Div Pulm & Crit Care Med, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 it Care Med, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Chem, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 , Dept Chem, St Louis, MO 63110 USA Howard Hughes Med Inst, St Louis, MO USA Howard Hughes Med Inst St Louis MO USA Hughes Med Inst, St Louis, MO USA
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 7, volume: 31, anno: 2001,
pagine: 1048 - 1059
SICI:
0954-7894(200107)31:7<1048:IDAMOU>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; BONE-MARROW PROGENITORS; NASAL EPITHELIAL-CELLS; MURINE MODEL; TH2 CELLS; TISSUE EOSINOPHILIA; CHEMOKINE EOTAXIN; INDUCED INCREASE; IGE PRODUCTION; RHINITIS;
Keywords:
allergic; inflammation; ovalbumin; eosinophil; mice; respiratory; olfactory;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Hamilos, DL Washington Univ, Sch Med, Div Allergy & Immunol, Box 8122,660 S Euclid Ave, St Louis, MO 63110 USA Washington Univ Box 8122,660 S Euclid Ave St Louis MO USA 63110
Citazione:
I. Hussain et al., "Induction, distribution and modulation of upper airway allergic inflammation in mice", CLIN EXP AL, 31(7), 2001, pp. 1048-1059

Abstract

Background To further elucidate mechanisms of human allergic rhinosinusitis, we studied the induction, distribution and modulation of allergen-induced upper airway inflammation in a BALB/c mouse model. Methods Allergic inflammation induced with ovalbumin (OVA) by intraperitoneal (IP) injection in alum was compared to repeated intranasal instillation. The type and distribution of inflammatory cells was compared in the respiratory and olfactory epithelial compartments. Eosinophil distribution was assessed using Scarlet Red stain and a polyclonal antibody recognizing eosinophil major basic protein (MBP). The role of interleukin (IL)-5 in upper airway inflammation was tested by administration of polyclonal anti-IL-5 antibody during the sensitization protocol. Results Unsensitized control mice receiving saline failed to develop upperairway eosinophil infiltration. IP OVA-sensitized mice developed marked upper airway mucosal eosinophil infiltration after aerosol OVA challenge, whereas repeated intranasal instillation of OVA produced qualitatively similar, but less intense eosinophil infiltration. Using either sensitization protocol, eosinophil infiltration was seen in areas of the lower portion of thenasal septum, the floor and the lower lateral walls of the mid-caudal region of the nasal cavity. Immunofluorescence staining for MBP confirmed this distribution of eosinophils but also demonstrated some eosinophils in the maxillary sinuses and in circumscribed regions of the ethmoturbinates. All areas of eosinophil infiltration were lined by respiratory epithelium. The selective infiltration of respiratory but not olfactory epithelium by eosinophils was unassociated with a measurable induction of epithelial ICA-M-1 oreotaxin expression. OVA-induced upper airway eosinophil infiltration was found to be IL-5 dependent, since administration of a polyclonal anti-IL-5 antibody (TRFK-5) during OVA sensitization resulted in a marked modulation (80% decrease) in eosinophil infiltration in response to subsequent OVA challenge. Conclusion The mouse upper airway, specifically in areas containing respiratory epithelium, is a target for OVA-induced allergic inflammation. This selective infiltration of respiratory, but not olfactory, epithelium is, in part, dependent upon IL-5. This model is useful for further dissection of the inflammatory response with genetic manipulations and targeted immunological approaches.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:53:52