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Titolo:
Filamentous bacteriophage stability in non-aqueous media
Autore:
Olofsson, L; Ankarloo, J; Andersson, PO; Nicholls, IA;
Indirizzi:
Univ Kalmar, Dept Chem & Biomed Sci, Bioorgan & Biophys Chem Lab, SE-39182Kalmar, Sweden Univ Kalmar Kalmar Sweden SE-39182 phys Chem Lab, SE-39182Kalmar, Sweden
Titolo Testata:
CHEMISTRY & BIOLOGY
fascicolo: 7, volume: 8, anno: 2001,
pagine: 661 - 671
SICI:
1074-5521(200107)8:7<661:FBSINM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR COAT PROTEIN; PHAGE-DISPLAY TECHNOLOGY; N-TERMINAL DOMAINS; CIRCULAR-DICHROISM; DETERGENT MICELLES; FORMING PROPERTIES; CRYSTAL-STRUCTURE; DNA PACKING; VIRUSES FD; I-FORMS;
Keywords:
organic solvent; capsid chemistry; circular dichroism; fluorescence; bacteriophage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Nicholls, IA Univ Kalmar, Dept Chem & Biomed Sci, Bioorgan & Biophys Chem Lab, SE-39182Kalmar, Sweden Univ Kalmar Kalmar Sweden SE-39182 , SE-39182Kalmar, Sweden
Citazione:
L. Olofsson et al., "Filamentous bacteriophage stability in non-aqueous media", CHEM BIOL, 8(7), 2001, pp. 661-671

Abstract

Background: Filamentous bacteriophage are used as general cloning vectors as well as phage display vectors in order to study li.-and-receptor interactions. Exposure to biphasic chloroform-water interface leads to specific contraction of phage, to noninfective I- or S-forms. Results: Upon exposure. phage were inactivated (non-infective) at methanol, ethanol and I-propanol concentrations inversely dependent upon alcohol hydrophobicity. Infectivity loss of phage at certain concentrations of I-propanol or ethanol coincided with changes in the spectral properties of the flvirion in ultraviolet fluorescence and circular dichroism studies. Conclusions: The alcohols inactivate filamentous phage by a general mechanism - solvation of coat protein - thereby disrupting the capsid in a mannerquite different from the previously reported I- and S-forms. The infectivity retention of phagemid pG8H6 in 99% acetonitrile and the relatively high general solvent resistance of the phage strains studied here open up the possibility of employing phage display in non-aqueous media. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 21:58:04