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Titolo:
Locating the metal ion in calcium-binding proteins by using cerium(III) asa probe
Autore:
Bertini, I; Lee, YM; Luchinat, C; Piccioli, M; Poggi, L;
Indirizzi:
Univ Florence, CERM, I-50019 Florence, Italy Univ Florence Florence Italy I-50019 ence, CERM, I-50019 Florence, Italy Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence FlorenceItaly I-50019 Dept Chem, I-50019 Florence, Italy Univ Florence, Dept Agr Biotechnol, I-50019 Florence, Italy Univ FlorenceFlorence Italy I-50019 Biotechnol, I-50019 Florence, Italy
Titolo Testata:
CHEMBIOCHEM
fascicolo: 7-8, volume: 2, anno: 2001,
pagine: 550 - 558
SICI:
1439-4227(20010803)2:7-8<550:LTMIIC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; N-TERMINAL DOMAIN; EF-HAND; PARAMAGNETIC METALLOPROTEINS; PSEUDOCONTACT SHIFTS; CALBINDIN D9K; STRUCTURE REFINEMENT; MOLECULAR-DYNAMICS; DIPOLAR COUPLINGS; NMR-SPECTROSCOPY;
Keywords:
calcium-binding proteins; cerium; metalloproteins; NMR spectroscopy; structure elucidation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Bertini, I Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy Univ Florence Via Luigi Sacconi 6 Florence Italy I-50019 Italy
Citazione:
I. Bertini et al., "Locating the metal ion in calcium-binding proteins by using cerium(III) asa probe", CHEMBIOCHEM, 2(7-8), 2001, pp. 550-558

Abstract

The detection and assignment of NMR spectroscopic signals of carbon atoms from carbonyl and carboxylate groups in the loop hosting the Ce-III ion wasperformed for the cerium-substituted calcium-binding protein calbindin D-9k. This provided a tool to characterize in solution the first coordination sphere of the metal ion. Due to the well-documented possibility of replacing calcium with metal ions of the Ln(III) series, this approach turns out tobe extremely efficient for characterizing in solution the coordination of calcium ions in proteins, independently of, the availability of X-ray crystal structures. The present approach completes the structural characterization of lanthanide-substituted calcium-binding proteins, for which the role of long-range constraints arising from hyperflne interaction and self-orientation has already been assessed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:40:05