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Titolo:
Calcium not strain regulates localization of alpha-myosin heavy chain mRNAin oriented cardiac myocytes
Autore:
Heidkamp, MC; Russell, B;
Indirizzi:
Univ Illinois, Coll Med, Dept Physiol & Biophys MC 901, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Biophys MC 901, Chicago, IL 60612 USA
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 305, anno: 2001,
pagine: 121 - 127
SICI:
0302-766X(200107)305:1<121:CNSRLO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; SKELETAL-MUSCLE; CONTRACTILE ACTIVITY; MECHANICAL-ACTIVITY; IN-VITRO; PROTEIN; HEART; MYOFIBRILLAR; EXPRESSION; TRANSLATION;
Keywords:
mechanical signal transduction; in situ hybridization; stretch; cell shape; rat (Sprague dawley);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Russell, B Univ Illinois, Coll Med, Dept Physiol & Biophys MC 901, 835 S Wolcott Ave,Chicago, IL 60612 USA Univ Illinois 835 S Wolcott Ave Chicago ILUSA 60612 60612 USA
Citazione:
M.C. Heidkamp e B. Russell, "Calcium not strain regulates localization of alpha-myosin heavy chain mRNAin oriented cardiac myocytes", CELL TIS RE, 305(1), 2001, pp. 121-127

Abstract

Our aim was to test a hypothesis that localization of the alpha -myosin heavy chain (alpha -MyHC) mRNA in oriented neonatal rat cardiomyocytes is regulated either by calcium, or by mechanical strain, or by both. Myocytes, grown on collagen aligned on stretchable silicone membranes, were elongated and had an increased length to width ratio (L/W) compared with randomly oriented myocytes grown on conventional substrata. Oriented cells were stretched by 10% in the longitudinal direction, in the transverse direction or passively unloaded for 6 h. As expected, shape changes followed these mechanical deformations. In situ hybridization was used to determine the localization of alpha -MyHC mRNA by quantitative analysis of optical density under various mechanical perturbations in myocytes that were either spontaneously beating or treated with verapamil (10 mM) to block influx of calcium. Unstretched, longitudinally stretched, and cells stretched transversely all had mRNA dispersed to their extremities. Verapamil treatment resulted in a perinuclear pattern of mRNA under all three mechanical perturbations. Additionally, mRNA distribution was examined in myocytes that were passively unloaded in the presence and absence of verapamil. Unloading myocytes with intact calcium cycling does not result in a perinuclear accumulation of mRNA. These data suggest that calcium is essential for alpha -MyHC mRNA distribution throughout the cell whereas stretch and alignment affect myocyte shape but have little effect on mRNA localization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 08:56:11