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Titolo:
Glial-cell-line-derived neurotrophic factor induces nerve fibre formation in primary cultures of adrenal chromaffin cells
Autore:
Forander, P; Broberger, C; Stromberg, I;
Indirizzi:
Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden Karolinska InstStockholm Sweden S-17177 osci, S-17177 Stockholm, Sweden
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 305, anno: 2001,
pagine: 43 - 51
SICI:
0302-766X(200107)305:1<43:GNFINF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICE LACKING GDNF; RET PROTOONCOGENE PRODUCT; NEURONS IN-VIVO; TYROSINE KINASE; POSTNATAL-DEVELOPMENT; DOPAMINERGIC-NEURONS; INSITU HYBRIDIZATION; SURVIVAL FACTOR; FIBER FORMATION; MESSENGER-RNA;
Keywords:
GDNF; TGF beta; GFR alpha; chromaffin cells; rat (Sprague dawley);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Stromberg, I Karolinska Inst, Dept Neurosci, Berzelius Vag 7,Plan 6, S-17177 Stockholm,Sweden Karolinska Inst Berzelius Vag 7,Plan 6 Stockholm Sweden S-17177
Citazione:
P. Forander et al., "Glial-cell-line-derived neurotrophic factor induces nerve fibre formation in primary cultures of adrenal chromaffin cells", CELL TIS RE, 305(1), 2001, pp. 43-51

Abstract

Neurotrophic factors, such as nerve growth factor (NGF), have been shown to promote the differentiation of neural crest neuroblasts into sympathetic neurons, whereas glucocorticoids promote the endocrine phenotype of adrenalmedullary chromaffin cells. This pluripotency is preserved to some extent in adult chromaffin cells, with NGF and other neurotrophic factors influencing the differentiation of these cells. In this study, the effects of glialcell line-derived neurotrophic factor (GDNF) on explanted chromaffin tissue have been investigated. The localization of mRNAs corresponding to the two components of the GDNF receptor, GDNF family receptor alpha 1 (GFR alpha1) and Ret, were demonstrated in adult adrenal medullary ganglion cells. GFRalpha1 mRNA was expressed in explanted chromaffin tissue at levels dependent on the presence of serum in the medium but decreased on the addition of blocking antibodies against transforming growth factor beta (TGF beta). However, TGF beta1 (1 ng/ml) did not upregulate GFR alpha1 mRNA expression when added to serum-free medium. GDNF induced neurite formation from chromaffin cells, as measured by the ratio of neurite-bearing versus total number ofchromaffin cells in primary cultures of adult adrenal medulla. The most potent dose inducing neurites from chromaffin cells was 100 ng/ml GDNF. However, this dose was not as efficient as that seen when chromaffin cells were stimulated with NGF (100 ng/ml). Thus, adrenal medullary cells express mRNAs for the GDNF receptor components Ret and GFR alpha1, increase their expression upon being cultured in serum-containing medium and respond to GDNF treatment with an increase in the number of cells that develop nerve processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:35:11