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Titolo:
Calbindin immunoreactivity of enteric neurons in the guinea-pig ileum
Autore:
Quinson, N; Robbins, HL; Clark, MJ; Furness, JB;
Indirizzi:
Univ Melbourne, Dept Anat & Cell Biol, Parkville, Vic 3010, Australia UnivMelbourne Parkville Vic Australia 3010 arkville, Vic 3010, Australia
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 305, anno: 2001,
pagine: 3 - 9
SICI:
0302-766X(200107)305:1<3:CIOENI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-BINDING PROTEINS; PRIMARY AFFERENT NEURONS; SMALL-INTESTINE; MYENTERIC NEURONS; SUBMUCOUS NEURONS; NERVOUS-SYSTEM; IMMUNOHISTOCHEMICAL EVIDENCE; SUBSTANCE-P; PROJECTIONS; PLEXUS;
Keywords:
enteric nervous system; sensory neuron; ileum; calbindin; guinea-pig;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Quinson, N Univ Melbourne, Dept Anat & Cell Biol, Parkville, Vic 3010, Australia Univ Melbourne Parkville Vic Australia 3010 ic 3010, Australia
Citazione:
N. Quinson et al., "Calbindin immunoreactivity of enteric neurons in the guinea-pig ileum", CELL TIS RE, 305(1), 2001, pp. 3-9

Abstract

Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferentneurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only calbindin-immunoreactive neurons in the plexus. Neurons with analogous shape and electrophysiology are found in submucosal ganglia, but, with antibodies used in previous studies, they lack calbindin immunoreactivity. An antiserum that is more effective in revealing calbindin in theguinea-pig enteric nervous system has been reported recently. In the present work, we found that this antiserum reveals the same population that was previously identified in myenteric ganglia, and does not reveal any furtherpopulation of myenteric nerve cells. In submucosal ganglia, 9-10% of nervecells were calbindin immunoreactive with this antiserum. The submucosal neurons with calbindin immunoreactivity were also immunoreactive for choline acetyltransferase, but not for neuropeptide Y (NPY) or vasoactive intestinal peptide (VIP). Small calbindin-immunoreactive neurons (average profile 130 mum(2)) were calretinin immunoreactive, whereas the large calbindin-immunoreactive neurons (average profile 330 mum(2)) had tachykinin (substance P)immunoreactivity. Calbindin immunoreactivity was seen in about 50% of the calretinin neurons and 40% of the tachykinin-immunoreactive submucosal neurons. It is concluded that, in the guinea-pig ileum, only one class of myenteric neuron, the AH/Dogiel type II neuron, is calbindin immunoreactive, but, in the submucosal ganglia, calbindin immunoreactivity occurs in cholinergic, calretinin-immunoreactive, secretornotor/vasodilator neurons and AH/Dogiel type II neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:17:39