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Titolo:
Enhanced growth suppression in esophageal carcinoma cells using adenovirus-mediated fusion gene transfer (uracil phosphoribosyl transferase and herpes simplex virus thymidine kinase)
Autore:
Shimizu, T; Shimada, H; Ochiai, T; Hamada, H;
Indirizzi:
Chiba Univ, Grad Sch Med, Acad Dept Surg, Chiba, Japan Chiba Univ Chiba Japan Univ, Grad Sch Med, Acad Dept Surg, Chiba, Japan Sapporo Med Univ, Dept Mol Med, Sapporo, Hokkaido, Japan Sapporo Med UnivSapporo Hokkaido Japan ol Med, Sapporo, Hokkaido, Japan
Titolo Testata:
CANCER GENE THERAPY
fascicolo: 7, volume: 8, anno: 2001,
pagine: 512 - 521
SICI:
0929-1903(200107)8:7<512:EGSIEC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE SUICIDE GENE; HUMAN COLON-CANCER; ESCHERICHIA-COLI; P53 GENE; RECOMBINANT ADENOVIRUS; COMBINATION THERAPY; IN-VIVO; 5-FLUOROURACIL; VECTOR; EXPRESSION;
Keywords:
uracil phosphoribosyltransferase; thymidine kinase; fusion gene; gene therapy; esophageal cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Shimada, H Chiba Univ, Sch Med, Dept Surg, Chuo Ku, 1-8-1 Inohana, Chiba 2608677, Japan Chiba Univ 1-8-1 Inohana Chiba Japan 2608677 ba 2608677, Japan
Citazione:
T. Shimizu et al., "Enhanced growth suppression in esophageal carcinoma cells using adenovirus-mediated fusion gene transfer (uracil phosphoribosyl transferase and herpes simplex virus thymidine kinase)", CANC GENE T, 8(7), 2001, pp. 512-521

Abstract

Advanced esophageal cancers are highly malignant and frequently resistant to 5-fluorouracil (5-FU). Escherichia coli uracil phosphoribosyltransferase(UP) is a pyrimidine salvage enzyme that alters 5-FU metabolism and sensitivity. A recombinant adenovirus encoding the UP gene (AxCA.UP) has been applied in gastric cancer gene therapy to sensitize cancer cells to lower concentrations of 5-FU. We have generated a recombinant adenovirus (AxCA.UT) encoding UP and herpes simplex virus thymidine kinase fusion protein (UT) to examine whether it would enhance the antitumor activity of AxCA.UP treatment. AxCA.UT treatment significantly enhanced the sensitivity of human esophageal cancer cells to and significantly enhanced the growth inhibition effects of UP gene therapy in vitro. Moreover, both 5-FU and ganciclovir showed bystander effects on growth inhibition. In an in vivo study, the therapeutic outcome of AxCA.UT treatment significantly enhanced the antitumor activity of AxCA.UP treatment. These observations suggest that AxCA.UT may be useful in esophageal cancer gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:49:55