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Titolo:
Natural killer cell-dependent apoptosis of peripheral murine hematopoieticprogenitor cells in response to Fas cross-linking: involvement of tumor necrosis factor-alpha
Autore:
Moreau, G; Leite-de-Moraes, M; Ezine, S; Di Santo, JP; Dy, M; Schneider, E;
Indirizzi:
Univ Paris 05, Hop Necker, CNRS, UMR 8603, F-75743 Paris 15, France Univ Paris 05 Paris France 15 , CNRS, UMR 8603, F-75743 Paris 15, France INSERM, U345, Paris, France INSERM Paris FranceINSERM, U345, Paris, France Inst Pasteur, Paris, France Inst Pasteur Paris FranceInst Pasteur, Paris, France
Titolo Testata:
BLOOD
fascicolo: 10, volume: 97, anno: 2001,
pagine: 3069 - 3074
SICI:
0006-4971(20010515)97:10<3069:NKCAOP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN NK CELLS; CD95 (APO-1/FAS)-MEDIATED APOPTOSIS; BONE-MARROW; MEDIATED APOPTOSIS; INTERFERON-GAMMA; IMMUNE-RESPONSES; EXPRESSION; RECEPTOR; CYTOTOXICITY; LIGAND;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schneider, E Univ Paris 05, Hop Necker, CNRS, UMR 8603, 161 Rue Sevres, F-75743 Paris 15, France Univ Paris 05 161 Rue Sevres Paris France 15 aris 15, France
Citazione:
G. Moreau et al., "Natural killer cell-dependent apoptosis of peripheral murine hematopoieticprogenitor cells in response to Fas cross-linking: involvement of tumor necrosis factor-alpha", BLOOD, 97(10), 2001, pp. 3069-3074

Abstract

Recently, a marked extramedullary myelopoiesis in Fas/CD95- or Fas/CD95L-deficient mice has been reported. In the present in vitro study, the mechanisms underlying Fas-Induced apoptosis of normal peripheral colony-forming unit-C (CFU-C) progenitors in the spleen were analyzed. Surprisingly, it was found that clonogenic progenitors were protected from gamma IFN plus Fas-induced programmed cell death when Lin(+) cells were removed from cultured splenocytes. The cells that rendered CFU-C sensitive to the activation of theFas pathway did not belong to the T or the myelocytic-monocytic lineage but comprised a non-B-cell subset expressing the activation marker B220. Among CD19(-) B220(+) splenocytes, nearly half were natural killer (NK) 1.1(+) cells whose in vivo depletion or deficiency in RAG2-gamma (-/-)(c) mice abrogated the effect of Fas cross-linking. NK cells exerted their accessory function, at least in part, through tumor necrosis factor-alpha. (TNF-alpha.), which they readily produced during pretreatment with the anti-Fas/CD95 monoclonal antibody and IFN-gamma and whose addition could compensate for theloss of sensitivity. In conclusion, this study provides evidence that peripheral clonogenic progenitors are not directly responsive to Fas cross-linking, even in the presence of IFN-gamma, but require NK cells as a source ofTNF-alpha. to make them susceptible to this death pathway. (Blood. 2001; 97:3069-3074) (C) 2001 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:10:30