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Titolo:
Physicochemical characteristics of LR3-IGF1 protein inclusion bodies: Electrophoretic mobility studies
Autore:
Wangsa-Wirawan, ND; ONeill, BK; Middelberg, APJ;
Indirizzi:
Univ Adelaide, Dept Chem Engn, Cooperat Res Ctr Tissue Growth & Repair, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 ir, Adelaide, SA 5005, Australia Univ Cambridge, Dept Chem Engn, Cambridge CB2 3RA, England Univ CambridgeCambridge England CB2 3RA ngn, Cambridge CB2 3RA, England
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 4, volume: 17, anno: 2001,
pagine: 786 - 790
SICI:
8756-7938(200107/08)17:4<786:PCOLPI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
FINE PARTICLES; FLOTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: O'Neill, BK Univ Adelaide, Dept Chem Engn, Cooperat Res Ctr Tissue Growth & Repair, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia5005 , SA 5005, Australia
Citazione:
N.D. Wangsa-Wirawan et al., "Physicochemical characteristics of LR3-IGF1 protein inclusion bodies: Electrophoretic mobility studies", BIOTECH PR, 17(4), 2001, pp. 786-790

Abstract

A knowledge of the physicochemical properties of inclusion bodies is important for the rational design of potential recovery processes such as flotation and precipitation. In this study, measurement of the size and electrophoretic mobility of protein inclusion bodies and cell debris was undertaken. SDS-PAGE analysis of protein inclusion bodies subjected to different cleaning regimes suggested that electrophoretic mobility provides a qualitative measure of protein inclusion body purity. Electrophoretic mobility as a function of electrolyte type and ionic strength was investigated. The presenceof divalent ions produced a stronger effect on electrophoretic mobility compared with monovalent ions. The isoelectric point of cell debris was significantly lower than that for the inclusion bodies. Hence, the contaminatingcell debris may be separated from inclusion bodies using flotation by exploiting this difference in isoelectric points. Separation by this method is simple, convenient, and a possible alternative to the conventional route ofcentrifugation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:08:36