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Titolo:
Scale-up of a high cell density continuous culture with Pichia pastoris X-33 for the constitutive expression of rh-chitinase
Autore:
Schilling, BM; Goodrick, JC; Wan, NC;
Indirizzi:
Genzyme Corp, Cambridge, MA 02139 USA Genzyme Corp Cambridge MA USA 02139Genzyme Corp, Cambridge, MA 02139 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 4, volume: 17, anno: 2001,
pagine: 629 - 633
SICI:
8756-7938(200107/08)17:4<629:SOAHCD>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FERMENTATION; YEAST; LYSOZYME; PROTEINS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Wan, NC Genzyme Corp, 1 Kendall Sq, Cambridge, MA 02139 USA Genzyme Corp 1Kendall Sq Cambridge MA USA 02139 dge, MA 02139 USA
Citazione:
B.M. Schilling et al., "Scale-up of a high cell density continuous culture with Pichia pastoris X-33 for the constitutive expression of rh-chitinase", BIOTECH PR, 17(4), 2001, pp. 629-633

Abstract

The feasibility of large-scale production of recombinant human chitinase using a constitutive Pichia pastoris expression system was demonstrated in a21-L continuous stirred tank reactor. A steady-state recombinant protein concentration of 250 mg/L in the supernatant was sustained for 1 month at a dilution rate of 0.042 h(-1) (equivalent to one volume exchange per day), enabling a volumetric productivity of 144 mg/L d (240 U/L d). The steady-state dry cell weight concentration in this high cell density culture reached 110 g/L. Considering safety and economical aspects, all large-scale cultivations were conducted without molecular oxygen supplementation. Conventionalair sparging was used instead. The oxygen demand of the process was determined by off-gas analysis (OUR = 4.8 g O-2 L-1 h(-1) with k(L)a = 846 h(-1))and evaluated with regard to further reactor scale-up.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:06:33