Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
High efficiencies of gene transfer with immobilized recombinant retrovirus: Kinetics and optimization
Autore:
Bajaj, B; Lei, P; Andreadis, ST;
Indirizzi:
SUNY Buffalo, Dept Chem Engn, Bioengn Lab, Amherst, NY 14260 USA SUNY Buffalo Amherst NY USA 14260 ngn, Bioengn Lab, Amherst, NY 14260 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 4, volume: 17, anno: 2001,
pagine: 587 - 596
SICI:
8756-7938(200107/08)17:4<587:HEOGTW>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; CATIONIC LIPOSOMES ENHANCE; APE LEUKEMIA-VIRUS; TARGET-CELLS; IN-VIVO; FIBRONECTIN FRAGMENTS; MAMMALIAN-CELLS; T-LYMPHOCYTES; HIGH-TITER; HALF-LIFE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Andreadis, ST SUNY Buffalo, Dept Chem Engn, Bioengn Lab, Amherst, NY 14260USA SUNY Buffalo Amherst NY USA 14260 ab, Amherst, NY 14260 USA
Citazione:
B. Bajaj et al., "High efficiencies of gene transfer with immobilized recombinant retrovirus: Kinetics and optimization", BIOTECH PR, 17(4), 2001, pp. 587-596

Abstract

We used a combination of mathematical modeling and experiments to investigate the rate-limiting steps of retroviral transduction. on surface-bound fibronectin (FN) and identify the conditions that maximize the efficiency of gene transfer. Our results show that fibronectin-assisted gene transfer (FAGT) is a strong function of the time and temperature of virus incubation inFN-coated plates. Gene transfer increases sharply at short times, reaches a maximum at intermediate times, and eventually declines as a result of loss of retroviral activity. The maximum transduction efficiency and the time at which this is attained increase with decreasing temperature of virus incubation. Depending on the temperature and the type of target cells, the initial rate of gene transfer increases by 3- to 10-fold and the maximum transduction efficiency increases by 2- to 4-fold as compared to traditional transduction (TT). Interestingly, Polybrene (PB) inhibits FAGT in a dose-dependent manner by inhibiting binding of retrovirus to FN. In contrast to traditional transduction, FAGT yields higher than 10-fold transduction efficiencies with concentrated retrovirus stocks. Gene transfer is directly proportional to the concentration of the virus-containing medium with no sign of saturation for the range of concentrations tested. These results suggest thatimmobilization of recombinant retrovirus can be rationally optimized to yield high efficiency of gene transfer to primary cells and improve the prospect of gene therapy for the treatment of human disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:42:23