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Titolo:
Tissue distribution and subcellular localization of a variant form of the human ST2 gene product, ST2V
Autore:
Tago, K; Noda, T; Hayakawa, M; Iwahana, H; Yanagisawa, K; Yashiro, T; Tominaga, S;
Indirizzi:
Jichi Med Sch, Dept Biochem, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Anat, Minami Kawachi, Tochigi 3290498, Japan Jichi MedSch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 5, volume: 285, anno: 2001,
pagine: 1377 - 1383
SICI:
0006-291X(20010803)285:5<1377:TDASLO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-1 RECEPTOR FAMILY; HELPER T-CELLS; ENDOTHELIAL-CELLS; EFFECTOR FUNCTION; RESPONSIVE GENE; PROMOTER USAGE; IL-1 RECEPTOR; EXPRESSION; T1/ST2; PROTEIN;
Keywords:
ST2 gene; interleukin 1 receptor family; membrane protein; immunoglobulin superfamily; ST2V protein; cytokine receptor; ligands; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Tominaga, S Jichi Med Sch, Dept Biochem, 3311-1 Yakushiji, Minami Kawachi,Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
K. Tago et al., "Tissue distribution and subcellular localization of a variant form of the human ST2 gene product, ST2V", BIOC BIOP R, 285(5), 2001, pp. 1377-1383

Abstract

The human ST2 gene has been known to encode three splice variants; namely,a soluble secreted form of ST2, a transmembrane form of ST2L, and ST2V of undetermined localization. Therefore, analysis of tissue distribution and subcellular localization of ST2V is important to elucidate functional relationships among the three splice variants of the human ST2 gene. RT-PCR procedure revealed that ST2V is predominantly expressed in the stomach, small intestine, and colon. Transfection of ST2V cDNA into COS7 cells in the presence of [S-35] methionine and cystein produced radiolabeled 40 kDa protein, which is recognized by specific monoclonal antibody against human ST2. Subcellular fractionation analysis showed that ST2V protein was distributed in the insoluble fraction of the cell lysate. Finally, ST2V protein was detected on the plasma membrane of COS7 cells, which had been transfected with ST2V cDNA, by confocal laser microscopic analysis. These findings taken together, indicate that ST2V protein localizes on the plasma membrane, suggestingits possible role in modification of the ST2L-signaling pathways. (C) 2001Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:29:34