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Titolo:
The role of amino acids in the regulation of hydrogen sulfide production during ultradian respiratory oscillation of Saccharomyces cerevisiae
Autore:
Sohn, HY; Kuriyama, H;
Indirizzi:
Inst Adv Ind Sci & Technol, Hokkaido Ctr, Toyohira Ku, Sapporo, Hokkaido 0628517, Japan Inst Adv Ind Sci & Technol Sapporo Hokkaido Japan 0628517 0628517, Japan
Titolo Testata:
ARCHIVES OF MICROBIOLOGY
fascicolo: 1-2, volume: 176, anno: 2001,
pagine: 69 - 78
SICI:
0302-8933(200107)176:1-2<69:TROAAI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTINUOUS-CULTURE; CYSTEINE BIOSYNTHESIS; TRANSCRIPTIONAL REGULATION; S-ADENOSYLMETHIONINE; SULFUR ASSIMILATION; YEAST; GLUTATHIONE; PATHWAY; ETHANOL; ACETYLTRANSFERASE;
Keywords:
cysteine; hydrogen sulfide; sulfate assimilation pathway; Saccharomyces cervisiae; ultradian respiratory oscillation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Sohn, HY Inst Adv Ind Sci & Technol, Hokkaido Ctr, Toyohira Ku, 17-2-1 Tsukisamu Higashi 2 Jo, Sapporo, Hokkaido 0628517, Japan Inst Adv Ind Sci & Technol 17-2-1 Tsukisamu Higashi 2 Jo Sapporo Hokkaido Japan 0628517
Citazione:
H.Y. Sohn e H. Kuriyama, "The role of amino acids in the regulation of hydrogen sulfide production during ultradian respiratory oscillation of Saccharomyces cerevisiae", ARCH MICROB, 176(1-2), 2001, pp. 69-78

Abstract

We previously demonstrated that periodic H2S production during aerobic continuous culture of Saccharomyces cerevisiae resulted in ultradian respiratory oscillation, and that H2S production was dependent on the activity of sulfate uptake and the level of sulfite. To investigate the mechanism of regulation of the sulfate assimilation pathway and of respiratory oscillation, several amino acids were pulse-injected into cultures during respiratory oscillation. Injection of sulfur amino acids or their derivatives perturbed respiratory oscillation, with changes in the H2S production profile. Four major regulators of H2S production in the sulfate assimilation pathway and respiratory oscillation were identified: (1) O-acetylhomoserine, not O-acetylserine, as a sulfide acceptor, (2) homoserine/ threonine as a regulator of O-acetylhomoserine supply, (3) methionine/S-adenosyl methionine as a negative regulator of sulfate assimilation, and (4) cysteine (or its derivatives)as an essential regulator. The results obtained after the addition Of DL-propargylglycine (5 muM and 100 muM) and cystathionine (50 muM) suggested that the intracellular cysteine level and cystathionine gamma -lyase, rather than methionine/S-adenosylmethionine, play an essential role in the regulation of sulfate assimilation and respiratory oscillation. Based on these results and those of our previous reports, we propose that periodic depletion of cysteine (or its derivatives), which is involved in the detoxification of toxic materials originating from respiration, causes periodic H2S production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:36:39