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Titolo:
uPA and uPAR contribute to NK cell invasion through the extracellular matrix
Autore:
Al-Atrash, G; Kitson, RP; Xue, YM; Mazar, AP; Kim, MH; Goldfarb, RH;
Indirizzi:
Univ N Texas, Ctr Hlth Sci, Dept Mol Biol & Immunol, Ft Worth, TX 76107 USA Univ N Texas Ft Worth TX USA 76107 Biol & Immunol, Ft Worth, TX 76107 USA Univ N Texas, Ctr Hlth Sci, Canc Res Inst, Ft Worth, TX 76107 USA Univ N Texas Ft Worth TX USA 76107 Canc Res Inst, Ft Worth, TX 76107 USA Angstrom Pharmaceut Inc, San Diego, CA USA Angstrom Pharmaceut Inc San Diego CA USA armaceut Inc, San Diego, CA USA
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 3B, volume: 21, anno: 2001,
pagine: 1697 - 1704
SICI:
0250-7005(200105/06)21:3B<1697:UAUCTN>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED KILLER-CELLS; ADOPTIVELY TRANSFERRED ADHERENT; UROKINASE PLASMINOGEN-ACTIVATOR; LARGE GRANULAR LYMPHOCYTES; RECOMBINANT INTERLEUKIN-2; INHIBITOR COMPLEXES; BASEMENT-MEMBRANE; LAK CELLS; LYMPHOKINE; RECEPTOR;
Keywords:
urokinase; plasminogen activator; natural killer cells; basement membrane; tumor infiltration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Goldfarb, RH Univ N Texas, Ctr Hlth Sci, Dept Mol Biol & Immunol, 3500 Camp Bowie Blvd,Ft Worth, TX 76107 USA Univ N Texas 3500 Camp Bowie Blvd Ft Worth TX USA 76107 7 USA
Citazione:
G. Al-Atrash et al., "uPA and uPAR contribute to NK cell invasion through the extracellular matrix", ANTICANC R, 21(3B), 2001, pp. 1697-1704

Abstract

Background: The urokinase plasminogen activator (uPA) system has been implicated in cellular invasiveness of tumor cells and immune cells. Herein we provide evidence for the production by natural killer (NK) cells of both uPA and its receptor (uPAR). Materials and Methods: Western blot analysis, RTPCR, casein/plasminogen zymography, and fluorescence microscopy were employed to detect uPA and uPAR on NK cells. NK cell invasiveness was examined using Matrigel invasion assays. Results: NK cell uPA appeared at its characteristic molecular weights, is enzymatically active in casein/plasminogen zymography, and is recognized by monoclonal antibodies. uPAR was detected by RTPCR and fluorescence microscopy. Matrigel invasion assays demonstrated an active role of uPA in NK cell invasion. Conclusion: The uPA system contributes to extracellular matrix (ECM) degradation by NK cells, which may be essential for NK cell accumulation into metastases, and may be prerequisite for their killing of tumor cells following NK cell adoptive transfer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:18:31