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Titolo:
Diagnostic application of CD44 variant expression in pancreatic juice for detection of pancreatic neoplasm
Autore:
Tsukuda, H; Matsumura, Y; Inoue, T; Kobayashi, Y; Ookawa, K; Aoki, T; Nebiki, H; Yamazaki, O; Kondo, H; Kakizoe, T;
Indirizzi:
Natl Canc Ctr Hosp, Dept Med, Chuo Ku, Tokyo 1040045, Japan Natl Canc Ctr Hosp Tokyo Japan 1040045 ed, Chuo Ku, Tokyo 1040045, Japan Osaka City Gen Hosp, Dept Pathol, Osaka, Japan Osaka City Gen Hosp OsakaJapan ity Gen Hosp, Dept Pathol, Osaka, Japan Osaka City Gen Hosp, Dept Endoscopy, Osaka, Japan Osaka City Gen Hosp Osaka Japan Gen Hosp, Dept Endoscopy, Osaka, Japan Osaka City Gen Hosp, Dept Surg, Osaka, Japan Osaka City Gen Hosp Osaka Japan City Gen Hosp, Dept Surg, Osaka, Japan
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 3C, volume: 21, anno: 2001,
pagine: 2175 - 2183
SICI:
0250-7005(200105/06)21:3C<2175:DAOCVE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
KI-RAS; DIFFERENTIAL EXPRESSION; HOMING RECEPTOR; GENE-MUTATIONS; CANCER; CELLS; ADENOCARCINOMA; EXONS; IDENTIFICATION; HYALURONATE;
Keywords:
CD44; pancreatic cancer; pancreatic juice and CD44 variants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Matsumura, Y Natl Canc Ctr Hosp, Dept Med, Chuo Ku, 1-1 Tsukiji 5 Chome, Tokyo 1040045,Japan Natl Canc Ctr Hosp 1-1 Tsukiji 5 Chome Tokyo Japan 1040045 n
Citazione:
H. Tsukuda et al., "Diagnostic application of CD44 variant expression in pancreatic juice for detection of pancreatic neoplasm", ANTICANC R, 21(3C), 2001, pp. 2175-2183

Abstract

Recently, increased and disorganized expression of CD44 variant exons (CD44v) has been demonstrated in several types of human malignancy. We tried toinvestigate CD44v expression in pancreatic juice from patients who underwent endoscopic retrograde pancreatography. We analyzed 24 patients with pancreatic neoplasms diagnosed histologically (adenocarcinoma, 17; adenoma, 7) and 15 patients with non-neoplastic lesions. The expression of CD44v mRNA in pancreatic juice was detected by using the reverse-transcription polymerase chain reaction technique followed by Southern hybridization with exon-specific probes. Of 17 patients with adenocarcinoma, 14 (82%) showed expression of CD44v6 mRNA and 11 (65%) showed expression of CD44v2 mRNA. Of 7 patients with adenoma, 6 (86%) were positive CD44v6 mRNA expression and 2 (29%) for CD44v2 mRNA expression; whiled out of 15 patients with non- neoplastic lesion, 5 (33%) showed positive findings for CD44V6 mRNA and 3 (20%) for CD44v2 mRNA. Comparing of diagnostic accuracy among CD44v6, CD44v2 and cytological examination, the sensitivities for adenocarcinoma were 82%, 65% and 41% respectively. However, the specificity was lower in CD44v6 (50%), CD44v2(77%) than in cytology (100%), because CD44v was positive in adenoma casesand normal cases. A combination of RT-PCR analysis for the expression of CD44v with cytological examination in the pancreatic juice, may increase theaccuracy of diagnosis for pancreatic cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 08:43:32