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Titolo:
Immunohistochemical localization of H-K-ATPase alpha(2c)-subunit in rabbitkidney
Autore:
Verlander, JW; Moudy, RM; Campbell, WG; Cain, BD; Wingo, CS;
Indirizzi:
Vet Affairs Med Ctr, Gainesville, FL 32608 USA Vet Affairs Med Ctr Gainesville FL USA 32608 r, Gainesville, FL 32608 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: F357 - F365
SICI:
0363-6127(200108)281:2<F357:ILOHAI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL COLLECTING DUCT; INTERCALATED CELLS; DISTAL NEPHRON; DEPLETION;
Keywords:
hypokalemia; anatomy; renal function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Wingo, CS Vet Affairs Med Ctr, Gainesville, FL 32608 USA Vet Affairs Med Ctr Gainesville FL USA 32608 ille, FL 32608 USA
Citazione:
J.W. Verlander et al., "Immunohistochemical localization of H-K-ATPase alpha(2c)-subunit in rabbitkidney", AM J P-REN, 281(2), 2001, pp. F357-F365

Abstract

The rabbit kidney possesses mRNA for the H-K-ATPase alpha (1)-subunit (HK alpha (1)) and two splice variants of the H-K-ATPase alpha (2)-subunit (HK alpha (2)). The purpose of this study was to determine the specific distribution of one of these, the H-K-ATPase alpha (2c)-subunit isoform (HK alpha (2c)), in rabbit kidney by immunohistochemistry. Chicken polyclonal antibodies against a peptide based on the NH2 terminus of HK alpha (2c) were used to detect HK alpha (2c) immunoreactivity in tissue sections. Immunohistochemical localization of HK alpha (2c) revealed intense apical immunoreactivity in a subpopulation of cells in the connecting segment, cortical collecting duct, and outer medullary collecting duct in both the outer and inner stripe. An additional population of cells exhibited a thin apical band of immunolabel. Immunohistochemical colocalization of HK alpha (2c) with carbonic anhydrase II, the Cl-/HCO3- exchanger AE1, and HK alpha (1) indicated that both type A and type B intercalated cells possessed intense apical HK alpha(2c) immunoreactivity, whereas principal cells and connecting segment cells had only a thin apical band of HK alpha (2c). Labeled cells were evident through the middle third of the inner medullary collecting duct in the majority of animals. Immunolabel was also present in papillary surface epithelial cells, cells in the cortical thick ascending limb of Henle's loop (cTAL), and the macula densa. Thus in the rabbit kidney, apical HK alpha (2c) is present and may contribute to acid secretion or potassium uptake throughoutthe connecting segment and collecting duct in both type A and type B intercalated cells, principal cells, and connecting segment cells, as well as incells in papillary surface epithelium, cTAL, and macula densa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 12:46:03