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Titolo:
Immunohistochemical localization of colonic H-K-ATPase to the apical membrane of connecting tubule cells
Autore:
Fejes-Toth, G; Naray-Fejes-Toth, A;
Indirizzi:
Dartmouth Coll Sch Med, Dept Physiol, Lebanon, NH 03756 USA Dartmouth CollSch Med Lebanon NH USA 03756 hysiol, Lebanon, NH 03756 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: F318 - F325
SICI:
0363-6127(200108)281:2<F318:ILOCHT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL COLLECTING DUCT; INTERCALATED CELLS; MONOCLONAL-ANTIBODY; MESSENGER-RNA; PEANUT LECTIN; RABBIT; EXPRESSION; TRANSPORT; KIDNEY; DEPRIVATION;
Keywords:
potassium transport; confocal microscopy; rabbit kidney;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Fejes-Toth, G Dartmouth Coll Sch Med, Dept Physiol, Lebanon, NH 03756 USA Dartmouth Coll Sch Med Lebanon NH USA 03756 n, NH 03756 USA
Citazione:
G. Fejes-Toth e A. Naray-Fejes-Toth, "Immunohistochemical localization of colonic H-K-ATPase to the apical membrane of connecting tubule cells", AM J P-REN, 281(2), 2001, pp. F318-F325

Abstract

Previous studies indicate that the colonic H-K-ATPase mRNA is expressed asthe distal nephron. However, the exact intrarenal localization of the colonic H-K-ATPase protein is still unclear. The goal of the present study was to determine the cellular and subcellular localization of the colonic H-K-ATPase protein in the rabbit kidney. We used three monoclonal antibodies (MAbs) directed against different epitopes of the rabbit colonic H-K-ATPase alpha -subunit (HK alpha (2)) to localize HK alpha (2) protein by immunofluorescence labeling of kidney sections and laser-scanning confocal microscopy. The specificity of the MAbs was confirmed by reaction with a single similar to 100-kDa band on Western blots of distal colon. Specific immunohistochemical reaction with the apical membrane of surface epithelial cells was observed with all three MAbs on distal colon sections. In rabbit kidney, immunofluorescence was detected only on the apical membrane of connecting tubulecells. Immunofluorescence was not detected in the cortical-, outer-, and inner-medullary collecting ducts. Furthermore, costaining with principal- and intercalated cell-specific MAbs and a MAb against the thick ascending limb suggests that these cell types express HK alpha2 protein at levels that are below the detection limit with this method. We conclude that in the rabbit kidney, under normal dietary conditions, the HK alpha2 protein is expressed in the apical membrane of connecting tubule cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:53:51