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Titolo:
Independent organic cation transport activity of Na+-L-carnitine cotransport system in LLC-PK1 cells
Autore:
Ohnishi, S; Saito, H; Fukada, A; Inui, KI;
Indirizzi:
Kyoto Univ Hosp, Dept Pharm, Fac Med, Sakyo Ku, Kyoto 6068507, Japan KyotoUniv Hosp Kyoto Japan 6068507 Med, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: F273 - F279
SICI:
0363-6127(200108)281:2<F273:IOCTAO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORDER-MEMBRANE-VESICLES; CATION/CARNITINE TRANSPORTER; KIDNEY-CELLS; OCTN2; LINE; CEPHALORIDINE; METABOLISM;
Keywords:
L-carnitine transporter; organic cation transporter; kidney epithelial cell line;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Inui, KI Kyoto Univ Hosp, Dept Pharm, Fac Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Hosp Kyoto Japan 6068507 yo Ku, Kyoto 6068507, Japan
Citazione:
S. Ohnishi et al., "Independent organic cation transport activity of Na+-L-carnitine cotransport system in LLC-PK1 cells", AM J P-REN, 281(2), 2001, pp. F273-F279

Abstract

We investigated expression of the Na+-L-carnitine cotransport system and its role in transport of tetraethylammonium in a kidney epithelial cell line, LLC-PK1. L-Carnitine uptake in the LLC-PK1 cells was markedly stimulated in the presence of Na+. The uptake was saturable, with Michaelis constant and maximal uptake velocity values of 7.8 muM and 153.7 pmol.mg protein(-1).15 min(-1), respectively. Cationic drugs such as tetraethylammonium, cimetidine, and quinidine inhibited L-carnitine uptake. The basolateral-to-apicaltransport of [C-14] tetraethylammonium was enhanced markedly in the presence of an H+ gradient on the apical side at a pH of 5.9. Under the conditions in which Na+/L-carnitine cotransport activity was saturable by the addition of 100 muM L-carnitine to the apical-side medium, the basolateral-to-apical transcellular transport of [C-14] tetraethylammonium was unaffected. These results suggested that the Na+-L-carnitine cotransporter is expressed in the apical membranes of LLC-PK1 cells, and is not responsible for efflux of tetraethylammonium from the cells. Transport of tetraethylammonium appeared to be mediated predominantly by an H+/ organic cation antiporter in theapical membranes.

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Documento generato il 03/07/20 alle ore 23:06:27