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Titolo:
Norepinephrine-induced calcium signaling pathways in afferent arterioles of genetically hypertensive rats
Autore:
Salomonsson, M; Arendshorst, WJ;
Indirizzi:
Univ N Carolina, Sch Med, Dept Cell & Mol Physiol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 ysiol, Chapel Hill, NC 27599 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: F264 - F272
SICI:
0363-6127(200108)281:2<F264:NCSPIA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; ANGIOTENSIN-II; SARCOPLASMIC-RETICULUM; INDUCED-CONTRACTIONS; RENAL VASCULATURE; CELLS; REACTIVITY; RELEASE; NORADRENALINE; RESISTANCE;
Keywords:
spontaneously hypertensive rats; Wistar-Kyoto rats; norepinephrine; hypertension; 8-(N,N-diethylamino) octyl-3,4,5-trimetoxybenzoate; nifedipine; vascular smooth muscle; inositol-1,4,5-trisphosphate-mediated mobilization; L-type calcium channel; renal circulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Arendshorst, WJ Univ N Carolina, Sch Med, Dept Cell & Mol Physiol, CB 7545,Rm 152,Med Sci Res Bldg, Chapel Hill, NC 27599 USA Univ N Carolina CB 7545,Rm 152,Med Sci Res Bldg Chapel Hill NC USA 27599
Citazione:
M. Salomonsson e W.J. Arendshorst, "Norepinephrine-induced calcium signaling pathways in afferent arterioles of genetically hypertensive rats", AM J P-REN, 281(2), 2001, pp. F264-F272

Abstract

This study provides new information about the relative importance of calcium mobilization and entry in the renal vascular response to adrenoceptor activation in afferent arterioles isolated from 7- to 8-wk-old Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Intracellular free calcium concentration ([Ca2+](i)) was measured in microdissected arterioles utilizing ratiometric photometry of fura 2 fluorescence. There was no significant strain difference in baseline [Ca2+](i). Norepinephrine (NE; 10(-6) and 10(-7) M) elicited immediate, sustained increases in [Ca2+](i). The general temporal pattern of response to 10(-6) M NE consisted of an initial peak and a maintained plateau phase. The response to NE was partially blocked by nifedipine (10(-6) M) or 8-( N,N-diethylamino) octyl-3,4,5-trimetoxybenzoate (TMB-8; 10(-5) M). A calcium-free external solution abolished the sustained [Ca2+](i) plateau response to NE, with less influence on the peak response. In the absence of calcium entry, TMB-8 (10(-5) M) completely blocked the calcium response to NE in WKY but not SHR, suggesting strain differences in mobilization. A higher concentration of TMB-8 (10(-4) M), however, blocked all discernible mobilization in both strains. We conclude that there are differences in Ca2+ handling in renal resistance vessels between young WKY and SHR with respect to mobilization stimulated by alpha -adrenoceptors. Afferent arterioles of young SHR appear to have a larger inositol-1,4,5-trisphosphate-sensitive pool or release from a site less accessible to TMB-8.

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Documento generato il 19/01/20 alle ore 12:12:55