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Titolo:
Heat shock protein 90 in retinal ganglion cells: Association with axonallytransported proteins
Autore:
Bernstein, SL; Russell, P; Wong, P; Fishelevich, R; Smith, LEH;
Indirizzi:
Univ Maryland, Sch Med, Dept Ophthalmol, Lab Mol Res, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Lab Mol Res, Baltimore, MD 21201 USA Childrens Hosp, Dept Ophthalmol, Boston, MA 02115 USA Childrens Hosp Boston MA USA 02115 Dept Ophthalmol, Boston, MA 02115 USA NEI, Lab Mech Ocular Dis, NIH, Bethesda, MD 20892 USA NEI Bethesda MD USA20892 ab Mech Ocular Dis, NIH, Bethesda, MD 20892 USA Univ Alberta, Dept Biol Sci, Edmonton, AB, Canada Univ Alberta Edmonton AB Canada rta, Dept Biol Sci, Edmonton, AB, Canada
Titolo Testata:
VISUAL NEUROSCIENCE
fascicolo: 3, volume: 18, anno: 2001,
pagine: 429 - 436
SICI:
0952-5238(200105/06)18:3<429:HSP9IR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; GROWTH-FACTOR; SIGNAL-TRANSDUCTION; VISUAL-SYSTEM; RAT RETINA; CONSTITUTIVE EXPRESSION; NEUROTROPHIN RECEPTORS; STEROID-RECEPTOR; TYROSINE KINASE; HSP90;
Keywords:
retinal ganglion cell; trkB; growth factor receptors; axon transport; MAP kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Bernstein, SL Univ Maryland, Sch Med, Dept Ophthalmol, Lab Mol Res, MSTF 5-65,10 S Pine St, Baltimore, MD 21201 USA Univ Maryland MSTF 5-65,10 S PineSt Baltimore MD USA 21201
Citazione:
S.L. Bernstein et al., "Heat shock protein 90 in retinal ganglion cells: Association with axonallytransported proteins", VIS NEUROSC, 18(3), 2001, pp. 429-436

Abstract

The mRNAs for heat shock protein 90 (HSP90) are found at highest levels (differentially expressed) in the primate retinal fovea, the region of highest visual acuity, compared to the peripheral retina. HSP90 expression and retinal associations were analyzed by immuno-localization, in situ hybridization, and western analysis. Retinal ganglion cells (RGCs) express much of the HSP90 mRNA present in the primate retinal fovea. A large fraction of RGC synthesized HSP90 is apparently present in the axonal compartment. To identify the role of HSP90 protein in the optic nerve and retina, co-immunoprecipitation experiments were performed, using antibodies specific for HSP90 isoforms. The immunoprecipitates were analyzed for neurotrophin receptor and ligand activities, and MAP kinase activity. MAP kinase assay was used to determine the activation state of MAP kinase associated with HSP90. HSP90 proteins selectively associate with the inactive form of full-length tyrosine kinase growth factor receptor trkB, suggesting utilization during anterograde axonal transport. Activated MAP kinase, associated with the trk downstream signaling cascade, was found to co-immunoprecipitate with optic nerve HSP90, suggesting that HSP90 may be utilized in retrograde transport of the secondary messengers associated with neurotrophin signaling. HSP90 can thus be hypothesized to play a role in bidirectional RGC axonal protein transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:00:16