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Titolo:
Localization and expression of osteopontin in the rotator cuff tendons in patients with calcifying tendinitis
Autore:
Takeuchi, E; Sugamoto, K; Nakase, T; Miyamoto, T; Kaneko, M; Tomita, T; Myoui, A; Ochi, T; Yoshikawa, R;
Indirizzi:
Osaka Univ, Sch Med, Dept Orthopaed Surg, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ed Surg, Suita, Osaka 5650871, Japan Hoshigaoka Koseinenkin Hosp, Dept Orthopaed Surg, Hirakata, Osaka 5730013,Japan Hoshigaoka Koseinenkin Hosp Hirakata Osaka Japan 5730013 ka 5730013,Japan Minoh City Hosp, Dept Orthopaed Surg, Minoh 5920014, Japan Minoh City Hosp Minoh Japan 5920014 Orthopaed Surg, Minoh 5920014, Japan
Titolo Testata:
VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY
fascicolo: 6, volume: 438, anno: 2001,
pagine: 612 - 617
SICI:
0945-6317(200106)438:6<612:LAEOOI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
URINARY STONE PROTEIN; SMOOTH-MUSCLE CELLS; MESSENGER-RNA; ATHEROSCLEROTIC PLAQUES; IN-VITRO; HYDROXYAPATITE FORMATION; SKELETAL TISSUES; CALCIFICATION; INHIBITION; MATRIX;
Keywords:
osteopontin; tendon; calcification; in situ hybridization; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Nakase, T Osaka Univ, Sch Med, Dept Orthopaed Surg, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 650871, Japan
Citazione:
E. Takeuchi et al., "Localization and expression of osteopontin in the rotator cuff tendons in patients with calcifying tendinitis", VIRCHOWS AR, 438(6), 2001, pp. 612-617

Abstract

Calcifying tendinitis of rotator cuff tendons is a common and painful condition caused by ectopic calcification in humans. To examine the involvementof osteopontin (OPN), a potent regulator of calcium deposition on connective tissues. localization and expression of OPN protein and messenger (m)RNAwere investigated in human tissue samples of calcified rotator cuff tendons. Immunohistochemistry demonstrated that OPN was localized in cells surrounding the calcified area. OPN was localized in two distinct cell types, i.e., fibroblast-like cells negative for CD68 and tartrate-resistant acid phosphatase (TRAP) and multinucleated macrophages positive for CD68 and TRAP. In situ hybridization revealed that the mRNA expression of OPN in these cells coincided with the immunohistochemistry results, and these results were supported by reverse transcriptase polymerase chain reaction analysis using human OPN-specific oligonucleotides. Cells located away from the calcified area did not express OPN. The present findings indicate the involvement of OPN in the process of calcification of rotator cuff tendons and suggest that OPN plays a role in such painful disorders through the actions of at least two cell types.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:31:55