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Titolo:
Quantitative and genotypic analysis of TT virus infection in Chinese blooddonors
Autore:
Zhong, S; Yeo, W; Lin, CK; Lin, XR; Tang, MW; Johnson, PJ;
Indirizzi:
Chinese Univ Hong Kong, Dept Clin Oncol, Sir YK Pao Ctr Canc, Prince WalesHosp, Shatin, Hong Kong, Peoples R China Chinese Univ Hong Kong Shatin Hong Kong Peoples R China Peoples R China Hong Kong Red Cross Blood Transfus Serv, Kowloon, Hong Kong, Peoples R China Hong Kong Red Cross Blood Transfus Serv Kowloon Hong Kong Peoples R China
Titolo Testata:
TRANSFUSION
fascicolo: 8, volume: 41, anno: 2001,
pagine: 1001 - 1007
SICI:
0041-1132(200108)41:8<1001:QAGAOT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEALTHY JAPANESE SUBJECTS; NON-A-HEPATITIS; DNA VIRUS; HIGH PREVALENCE; NONHUMAN-PRIMATES; LIVER-DISEASE; SEQUENCES; TRANSMISSION; EXCRETION; HUMANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, PJ Chinese Univ Hong Kong, Dept Clin Oncol, Sir YK Pao Ctr Canc, Prince WalesHosp, Shatin, Hong Kong, Peoples R China Chinese Univ Hong Kong Shatin Hong Kong Peoples R China hina
Citazione:
S. Zhong et al., "Quantitative and genotypic analysis of TT virus infection in Chinese blooddonors", TRANSFUSION, 41(8), 2001, pp. 1001-1007

Abstract

BACKGROUND: The TT virus (TTV) is a member of a newly described family of human viruses related to the Circoviridae viruses. Its association with specific diseases has not been established, and screening of blood donors has not been implemented. To date, 16 genotypes have been identified. STUDY DESIGN AND METHODS: Sera from 471 healthy blood donors (aged 11-58 years) were randomly selected and tested for TTV by the use of two sets of primers: NG59d/NG61d/NG63d primers and T801/T935 primers. Quantitative competitive PCR (QC-PCR) was developed to measure the TTV DNA concentration among the blood donors. Sequencing of a part of the genome was performed to identify the various genotypes. Several samples showed a mixed genotype infection. RESULTS: TTV was detected in 251 (53.3%) of 471 healthy Hong Kong blood donors by the use of NG59d/NG61d/NG63d primers. The prevalence of the virus increased steadily with age (p = 0.03). TTV DNA was detected in 90 percent (90 of a randomly selected 100) of samples by the use of T801/T935 primers. TTV DNA concentration was also measured by QC-PCR in the blood donors who were positive for TTV DNA in the first round of the heminested PCR. TTV titers ranged from 4.8 x 10(2) copies per mL to 6 x 10(4) Copies per mL, with amedian value of 1.2 x 10(4) copies per mL. Sequencing and phylogenetic analysis of a 223-bp fragment from open reading frame 1 showed three main genotypes (G1 [60.7%], G2 [24.3%], and G3 [14%]) and a new genotype 17 (G17), with the latter bearing 60-percent nucleotide homology with other genotypes deposited at GenBank. In addition, a new TTV subtype, G2f, was found. CONCLUSION: The prevalence of TTV is high in healthy Chinese blood donors. Three main genotypes (G1, G2, and G3) were detected. In addition, a new TTV genotype, tentatively designated as G17, and a new subtype, G2f, were identified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:00:59