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Titolo:
Masculinization of female mosquitofish in kraft mill effluent-contaminatedFenholloway River water is associated with androgen receptor agonist activity
Autore:
Parks, LG; Lambright, CS; Orlando, EF; Guillette, LJ; Ankley, GT; Gray, LE;
Indirizzi:
US EPA, NHEERL, RTD, Endocrinol Branch, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA 27711 Branch, Res Triangle Pk, NC 27711 USA Univ Florida, Dept Zool, Gainesville, FL 32611 USA Univ Florida Gainesville FL USA 32611 ept Zool, Gainesville, FL 32611 USA St Marys Coll, St Marys City, MD USA St Marys Coll St Marys City MD USASt Marys Coll, St Marys City, MD USA US EPA, NHEERL, Mid Continent Ecol Div, Duluth, MN USA US EPA Duluth MN USA EPA, NHEERL, Mid Continent Ecol Div, Duluth, MN USA
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 2, volume: 62, anno: 2001,
pagine: 257 - 267
SICI:
1096-6080(200108)62:2<257:MOFMIK>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
BENIGN PROSTATIC HYPERPLASIA; DEPENDENT GENE-EXPRESSION; RAINBOW-TROUT; MALE-RAT; VINCLOZOLIN; TESTOSTERONE; STEROLS; DIFFERENTIATION; ANTIANDROGEN; REPRODUCTION;
Keywords:
environmental androgens; Kraft pulp and paper mill effluent; masculinized female mosquitofish anal fin; gonopodium; androgen receptor; glucocorticoid receptor; AR binding; AR gene expression; in vitro; Fenholloway River; Florida;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Gray, LE US EPA, NHEERL, RTD, Endocrinol Branch, MD-72, Res Triangle Pk, NC 27711 USA US EPA MD-72 Res Triangle Pk NC USA 27711 iangle Pk, NC 27711 USA
Citazione:
L.G. Parks et al., "Masculinization of female mosquitofish in kraft mill effluent-contaminatedFenholloway River water is associated with androgen receptor agonist activity", TOXICOL SCI, 62(2), 2001, pp. 257-267

Abstract

Female mosquitofish (Gambusia affinis holbrooki) downstream from Kraft paper mills in Florida display masculinization of the anal fin, an androgen-dependent trait. The current investigation was designed to determine if water contaminated with pulp-mill effluent (PME) from the Fenholloway River in Florida displayed androgenic activity in vitro and to relate this activity to the reproductive status of female mosquitofish taken from this river. Wetested water samples for androgenic activity from a reference site upstream of a Kraft pulp and paper mill on the Fenholloway River, from 3 sites downstream from the mill, and from another reference site on the Econfina. River, also in Florida, where there is no paper mill. We also examined anal fin ray morphology in mosquitofish from these rivers for evidence of masculinization. Eighty percent of the female mosquitofish from the Fenholloway River were partially masculinized while another 10% were completely masculinized, based upon the numbers of segments in the longest anal fin ray (18.0 +/- 0.4 vs. 28.1 +/- 0.9 [p < 0.001]) in the Econfina River vs. the Fenholloway River, respectively). In a COS whole cell-binding assay, all 3 PME samples displayed affinity for human androgen receptor (hAR) (p < 0.001). In addition, PME induced androgen-dependent gene expression in CV-1 cells (cotransfected with pCMV hAR and MMTV luciferase reporter), which was inhibited byabout 50% by coadministration of hydroxyflutamide (1 muM), an AR antagonist. Water samples collected upstream of the Kraft mill or from the Econfina River did not bind hAR or induce luciferase expression. When CV-1 cells were transfected with human glucocorticoid receptor (hGR) rather than hAR, PMEfailed to significantly induce MMTV-luciferase expression. Further evidence of the androgenicity was observed using a COS cell AR nuclear-translocalization assay. PME bound hAR and induced translocalization of AR into the nucleus. In contrast, AR remained perinuclear when treated with water from the control sites (indicating the absence of an AR ligand). Interestingly, PME also displayed "testosterone-like" immunoreactivity in a testosterone radioimmunoassay, whereas water from the reference sites did not. In summary, water collected downstream of the Kraft mill on the Fenholloway River contains unidentified androgenic substances whose presence is associated with masculinization of female mosquitofish.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:00:37