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Titolo: Total regional and global number of synapses in the human brain neocortex
Autore: Tang, Y; Nyengaard, JR; De Groot, DMG; Gundersen, HJG;
 Indirizzi:
 Aarhus Univ, Inst Expt Clin Res, Stereol Res Lab, DK8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C Stereol Res Lab, DK8000 Aarhus C, Denmark Aarhus Univ, Inst Expt Clin Res, Electron Microscopy Lab, DK8000 Aarhus, Denmark Aarhus Univ Aarhus Denmark DK8000 croscopy Lab, DK8000 Aarhus, Denmark Kommune Hosp Copenhagen, Bartholin Inst, Neurol Res Lab, Copenhagen, Denmark Kommune Hosp Copenhagen Copenhagen Denmark Res Lab, Copenhagen, Denmark TNO, Nutr & Food Res Inst, Dept Target Organ Toxicol, NL3700 AJ Zeist, Netherlands TNO Zeist Netherlands NL3700 AJ Toxicol, NL3700 AJ Zeist, Netherlands
 Titolo Testata:
 SYNAPSE
fascicolo: 3,
volume: 41,
anno: 2001,
pagine: 258  273
 SICI:
 08874476(200109)41:3<258:TRAGNO>2.0.ZU;23
 Fonte:
 ISI
 Lingua:
 ENG
 Soggetto:
 UNBIASED STEREOLOGICAL ESTIMATION; SEGMENTED POSTSYNAPTIC DENSITIES; LONGTERM POTENTIATION; RAT DENTATE GYRUS; NUMERICAL DENSITY; AXOSPINOUS SYNAPSES; QUANTITATIVE STEREOLOGY; PERFORATED SYNAPSES; ARBITRARY PARTICLES; PLASTICITY;
 Keywords:
 human brain; neocortex; number; stereology; synapse;
 Tipo documento:
 Article
 Natura:
 Periodico
 Settore Disciplinare:
 Life Sciences
 Citazioni:
 52
 Recensione:
 Indirizzi per estratti:
 Indirizzo: Nyengaard, JR Aarhus Univ, Inst Expt Clin Res, Stereol Res Lab, Bldg 182, DK8000 AarhusC, Denmark Aarhus Univ Bldg 182 Aarhus Denmark C 8000 AarhusC, Denmark



 Citazione:
 Y. Tang et al., "Total regional and global number of synapses in the human brain neocortex", SYNAPSE, 41(3), 2001, pp. 258273
Abstract
An estimator of the total number of synapses in neocortex. of human autopsy brains based on unbiased stereological principles is described. Each randomly chosen cerebral hemisphere was stratified into the four major neocortical regions. Uniform sampling with a varying sampling fraction in each region of neocortex was performed. The total volume of each neocortical region was estimated using point counting according to Cavalieri's principle. The ethanolic phosphotungstic acid staining technique was modified for synapsesin human autopsy brains. The numerical density of synapses in each neocortical region studied was estimated using the disector at the electron microscopical level. The total number of neocortical synapses in each region was estimated as the product of the total volume of neocortex. and the numerical density of synapses. The influence of the postmortem fixation delay on the number of synapses was investigated in five large mammals (one dog, one cow, and three pigs), the brains of which were kept under conditions similarto those under which human corpses are normally kept. The apparent decrease of 3.9% in the numerical density of synapses in the large mammals following a 2day fixation delay was not significant. The average total number of synapses in the neocortex of five young male brains was 164 x 10(12) (CV = 0.17). An analysis of the precision of the estimate of the total number of synapses in neocortex indicates that blocks represent both the major sourceof variation and the largest workload. Using eight blocks per brain the imprecision of the estimate is, however, only 66% of the total variance. Synapse 41:258273, 2001. (C) 2001 WileyLiss, Inc.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 07:57:11