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Titolo:
Changes in cannabinoid CB1 receptors in striatal and cortical regions of rats with experimental allergic encephalomyelitis, an animal model of multiple sclerosis
Autore:
Berrendero, F; Sanchez, A; Cabranes, A; Puerta, C; Ramos, JA; Garcia-Merino, A; Fernandez-Ruiz, J;
Indirizzi:
Univ Complutense, Fac Med, Dept Bioquim & Biol Mol, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 & Biol Mol, E-28040 Madrid, Spain Hosp Univ Puerta Hierro, Lab Neuroinmunol, Madrid 28035, Spain Hosp Univ Puerta Hierro Madrid Spain 28035 oinmunol, Madrid 28035, Spain
Titolo Testata:
SYNAPSE
fascicolo: 3, volume: 41, anno: 2001,
pagine: 195 - 202
SICI:
0887-4476(200109)41:3<195:CICCRI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; MESSENGER-RNA; BRAIN-DEVELOPMENT; BASAL GANGLIA; LEWIS RATS; LOCALIZATION; ANANDAMIDE; SYSTEM; AUTORADIOGRAPHY; HYBRIDIZATION;
Keywords:
CB1 receptors; cannabinoids; multiple sclerosis; EAE; brain regions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Fernandez-Ruiz, J Univ Complutense, Fac Med, Dept Bioquim & Biol Mol, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 40 Madrid, Spain
Citazione:
F. Berrendero et al., "Changes in cannabinoid CB1 receptors in striatal and cortical regions of rats with experimental allergic encephalomyelitis, an animal model of multiple sclerosis", SYNAPSE, 41(3), 2001, pp. 195-202

Abstract

Data, initially anecdotal, but recently supported on more solid experimental evidence, suggest that cannabinoids might be beneficial in the treatmentof some of the symptoms of multiple sclerosis (MS). Despite this evidence,there are no data on the possible changes in cannabinoid CB1 or CB2 receptors, the main molecular targets for the action of cannabinoids, either in the postmortem brain of patients with MS or in animal models of this disease. The present study addressed this question using the model of experimentalallergic encephalomyelitis (EAE) in Lewis rats generated by inoculation ofguinea pig myelin basic protein in Freund's adjuvant. After inoculation, animals were examined daily to detect the appearance of neurological signs. The first signs appeared around day 10 after inoculation, reaching the highest degree by day 13, when animals were sacrificed and their brains removedand used for analysis of CB1 receptor binding, mRNA levels, and activationof GTP-binding proteins. CB1 receptor binding and mRNA levels were not affected in EAE rats in brain areas such as the hippocampus, limbic structures, and cerebellum. However, there was a marked decrease in both parameters in the caudate-putamen, both in the lateral and medial parts, although this decrease did not correspond with decreases in binding in the nuclei recipient of striatal output neurons, which suggests that changes in CB1 receptorsare exclusively located in the cell bodies of striatal neurons. In addition, CB1 receptor binding, but not mRNA levels, also decreased in the cerebral cortex, both in the deep and the superficial layers. The analysis of [S-35]GTP gammaS binding after activation of CB1 receptors with WIN55,212-2, a synthetic agonist, revealed that, despite the decrease in the number of CB1receptors in EAE rats, these were more efficiently coupled to GTP-binding protein-mediated signaling mechanisms in both the caudate-putamen and the cerebral cortex of these animals. In summary, these data suggest that the generation of EAE in Lewis rats would be associated with changes in CB1 receptors in striatal and cortical neurons, which might be related to the alleviation of some motor signs observed after the treatment with cannabinoid receptor agonists in similar models of MS in rodents. Synapse 41:195-202, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:10:47