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Titolo:
Short term maintenance of sheep preantral follicles in situ in 0.9% salineand Braun-Collins solution
Autore:
Andrade, ER; Rodrigues, APR; Amorim, CA; Carvalho, FCA; Dode, MAN; Figueiredo, JR;
Indirizzi:
State Univ Ceara, Fac Vet, Programa Posgrad Ciencias Vet, LAMOFOPA, BR-60740000 Fortaleza, Ceara, Brazil State Univ Ceara Fortaleza Ceara Brazil BR-60740000 BCleza, Ceara, Brazil Univ Santa Maria, BR-97105900 Santa Maria, RS, Brazil Univ Santa Maria Santa Maria RS Brazil BR-97105900 BCta Maria, RS, Brazil Natl Ctr Res Beef Cattle, BR-79002970 Campo Grande, MS, Brazil Natl Ctr Res Beef Cattle Campo Grande MS Brazil BR-79002970 BC MS, Brazil
Titolo Testata:
SMALL RUMINANT RESEARCH
fascicolo: 2, volume: 41, anno: 2001,
pagine: 141 - 149
SICI:
0921-4488(200108)41:2<141:STMOSP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNIVERSITY-OF-WISCONSIN; ISCHEMIC TISSUE INJURY; IN-VITRO; EURO-COLLINS; BOVINE OOCYTES; PRESERVATION; ATRESIA; POPULATION; STORAGE; OVARIES;
Keywords:
sheep; preantral follicle; preservation; histology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Andrade, ER State Univ Ceara, Fac Vet, Programa Posgrad Ciencias Vet, LAMOFOPA, Av Paranjana,1700 Campus Itaperi, BR-60740000 Fortaleza, Ceara, Brazil State Univ Ceara Av Paranjana,1700 Campus Itaperi Fortaleza Ceara Brazil BR-60740000 BC
Citazione:
E.R. Andrade et al., "Short term maintenance of sheep preantral follicles in situ in 0.9% salineand Braun-Collins solution", SMALL RUMIN, 41(2), 2001, pp. 141-149

Abstract

The present study has investigated the efficiency of 0.9% saline solution and Braun-Collins solution in the preservation of sheep preantral folliclesin situ, at different temperatures and different incubation periods. The ovaries were divided into 19 fragments for each animal. One ovarian fragmentwas taken randomly and immediately fixed for histology (control-time zero). The other 18 ovarian fragments were randomly distributed in tubes containing 2 ml of 09% saline solution or Braun-Collins solution at 4, 20 or 39 degreesC for 4, 12 or 24 h. A total of 1629, 208 and 63 primordial, primary and secondary follicles were examined, respectively. Histological analysis showed that the storage of ovarian fragments in both solutions at 20 or 39 degreesC significantly reduced the percentage of morphologically normal preantral follicles (MNPF) when compared with the control, except after preservation in 0.9% saline solution at 20 degreesC for 4 h. In contrast, the preservation at 4 degreesC, in both solutions. kept the percentage of MNPF similar to control values. In conclusion, this study shows for the first time that sheep preantral follicles can be preserved in situ successfully at 4 degreesC in 0.9% saline solution or Braun-Collins solution for up to 24 h. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:53:44