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Titolo:
Ex vivo fluorescence microscopy provides simple and accurate assessment ofneutrophil-endothelial adhesion in the rat lung
Autore:
Han, I; Saito, H; Fukatsu, K; Inoue, T; Yasuhara, H; Furukawa, S; Matsuda, T; Lin, MT; Ikeda, S;
Indirizzi:
Univ Tokyo, Fac Med, Surg Ctr, Bunkyo Ku, Tokyo 1138655, Japan Univ TokyoTokyo Japan 1138655 Surg Ctr, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo, Fac Med, Dept Surg, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 ept Surg, Bunkyo Ku, Tokyo 1138655, Japan
Titolo Testata:
SHOCK
fascicolo: 2, volume: 16, anno: 2001,
pagine: 143 - 147
SICI:
1073-2322(200108)16:2<143:EVFMPS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE-ORGAN FAILURE; SYSTEMIC INFLAMMATORY RESPONSE; PULMONARY MICROCIRCULATION; ACUTE-PANCREATITIS; SEPTIC SHOCK; RABBIT LUNG; ENDOTOXIN; INJURY; ACTIVATION; LEUKOCYTES;
Keywords:
lipopolysaccharide; visualization; quantification; ICAM-1; beta 2-integrin; myeloperoxidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Saito, H Univ Tokyo, Fac Med, Surg Ctr, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 Tokyo 1138655, Japan
Citazione:
I. Han et al., "Ex vivo fluorescence microscopy provides simple and accurate assessment ofneutrophil-endothelial adhesion in the rat lung", SHOCK, 16(2), 2001, pp. 143-147

Abstract

Neutrophil adhesion to the pulmonary endothelium Is prerequisite to neutrophil transmigration and activation, both of which may lead to lung Injury, A simple method to evaluate neutrophil adherence in the lung would be useful for developing new strategies for neutrophil-mediated lung Injury. The purpose was to establish a simple method to evaluate neutrophil adhesion in the lung using ex vivo fluorescence microscopy. Rats were anesthetized, aridthe right jugular veins were catheterized. Neutrophils were isolated from another set of rats and labeled with 5,(6)-carboxyfluorescein diacetate. Animals were killed 120 s after a 1 x 10(6) labeled neutrophil injection. Thepulmonary labeled neutrophil number was counted under a fluorescence microscope. In the first experiment, rats were given 0, 20, 200, or 2000 pg/kg lipopolysaccharide (LPS) Lp. At 4 h after challenge, the pulmonary labeled neutrophil number was determined. Kinetic studies were also performed at 0, 1, 4, and 8 h after 200 pg/kg LPS. Finally, anti-ICAM-1 Ab was injected im.before LPS 200 pg/kg, and the labeled neutrophil number in the lung was determined at 4 h. The number of pulmonary labeled neutrophils. was higher after LPS 200 or 2000 pg/kg than after the other doses. The pulmonary labeledneutrophil number was increased at 4 h compared with the other time points. ICAM-1 blocking normalized the pulmonary labeled neutrophil number in theLPS group. In conclusion, our method seems to reflect ICAM-1-mediated neutrophil adherence to the endothelium In the present setting. This simple technique may be useful for evaluating neutrophil adhesion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:52:54