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Titolo:
Strand breaks in whole plasmid DNA produced by the decay of I-125 in a triplex-forming oligonucleotide
Autore:
Panyutin, IV; Luu, AN; Panyutin, IG; Neumann, RD;
Indirizzi:
NIH, Warren G Magnuson Clin Ctr, Dept Nucl Med, Bethesda, MD 20854 USA NIH Bethesda MD USA 20854 Clin Ctr, Dept Nucl Med, Bethesda, MD 20854 USA
Titolo Testata:
RADIATION RESEARCH
fascicolo: 2, volume: 156, anno: 2001,
pagine: 158 - 166
SICI:
0033-7587(200108)156:2<158:SBIWPD>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-EMITTING I-125; SYNTHETIC OLIGODEOXYNUCLEOTIDE; MAMMALIAN-CELLS; LABELED OLIGONUCLEOTIDES; QUANTITATIVE-ANALYSIS; POSITIONAL CHANGES; AUGER; RADIOTOXICITY; I125; RADIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Panyutin, IV NIH, Warren G Magnuson Clin Ctr, Dept Nucl Med, Bld 10,Room 4D43,10 Cent Dr, Bethesda, MD 20854 USA NIH Bld 10,Room 4D43,10 Cent Dr Bethesda MD USA 20854 854 USA
Citazione:
I.V. Panyutin et al., "Strand breaks in whole plasmid DNA produced by the decay of I-125 in a triplex-forming oligonucleotide", RADIAT RES, 156(2), 2001, pp. 158-166

Abstract

DNA strand breaks produced by the decay of I-125 positioned against a specific site in plasmid DNA via a triplex-forming oligonucleotide were studiedboth in the immediate vicinity of the site of the decay with a single nucleotide resolution and in the whole plasmid by measuring the percentages of supercoiled, open-circular and linear forms. The localized breaks are distributed within 10 bp in each direction from the decay site with maxima in both strands just opposite the I-125-dC residue in the triplex-forming oligonucleotide. The distributions of breaks in the two DNA strands are almost symmetrical, in agreement with the geometry of the pyrimidine motif triplex. We found that about 25% of the double-strand breaks were located outside the 90-bp fragment containing the triplex-forming oligonucleotide binding sequence. The ratio of single-to double-strand breaks in the whole plasmid was11 for bound triplex-forming oligonucleotide compared to 26 when the triplex-forming oligonucleotide was free in solution. The number of double-strand breaks per decay of I-125 was 0.46 for bound triplex-forming oligonucleotide and 0.17 for free triplex-forming oligonucleotide. Comparing the data on the localized damage and those for the whole plasmid, we concluded that, in addition to DNA breaks that are confined to a helical turn around the I-125 atom, the decay can produce breaks hundreds of base pairs away in the plasmid molecule. This linear plasmid molecule containing radiation-induced damage at a specific DNA site should be useful in studies of the molecular mechanisms of DNA repair. (C) 2001 by Radiation Research Society.

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Documento generato il 26/09/20 alle ore 06:56:07