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Titolo:
Structural rationale for the modulation of abscess formation by Staphylococcus aureus capsular polysaccharides
Autore:
Tzianabos, AO; Wang, JY; Lee, JC;
Indirizzi:
Harvard Univ, Brigham & Womens Hosp, Sch Med, Channing Lab,Dept Mtd, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 nning Lab,Dept Mtd, Boston, MA 02115 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 16, volume: 98, anno: 2001,
pagine: 9365 - 9370
SICI:
0027-8424(20010731)98:16<9365:SRFTMO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL INTRAABDOMINAL SEPSIS; BACTEROIDES-FRAGILIS; MICROCAPSULE EXPRESSION; BACTERIAL VIRULENCE; TYPE-5; CELLS; ANTIBODIES; PROTECTION; VACCINES; MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Tzianabos, AO Channing Labs, 181 Longwood Ave, Boston, MA 02115 USA Channing Labs 181 Longwood Ave Boston MA USA 02115 02115 USA
Citazione:
A.O. Tzianabos et al., "Structural rationale for the modulation of abscess formation by Staphylococcus aureus capsular polysaccharides", P NAS US, 98(16), 2001, pp. 9365-9370

Abstract

Staphylococcus aureus is a medically important bacterial pathogen that is a common cause of superficial and deep-seated abscesses in humans. Most S. aureus isolates produce either a serotype 5 or 8 capsular polysaccharide (CP) that has been shown to enhance bacterial virulence. We investigated the role of 5. aureus CPs in modulating abscess formation in an experimental animal model of intraabdominal infection. Structural studies of CP8 revealed that it has a zwitterionic charge motif conferred by the negatively chargedcarboxyl group of N-acetylmannosaminuronic acid and free amino groups available on partially N-acetylated fucosamine residues. We report that purified CP5 and CP8 facilitated intraabdominal abscess formation in animals when given i.p. with a sterile cecal contents adjuvant. Chemical modifications that neutralized the positively or negatively charged groups on CP8 abrogated its ability to provoke abscesses. Rats prophylactically treated with CP8 s.c. were protected against abscess formation induced by homologous or heterologous zwitterionic polysaccharides. Likewise, treatment with CP8 protected against challenge with viable S. aureus strains PS80 (a capsule type 8 strain) or COL (a methicillin-resistant capsule type 5 strain). Purified CP8was a potent activator of rat and human CD4(+) T cells in vitro. When transferred to naive rats, these activated T cells modulated the development ofintraabdominal abscess formation. These results provide a structure/function rationale for abscess formation by S, aureus and expand the sphere of encapsulated organisms that interact directly with T cells to regulate this host response to bacterial infection.

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Documento generato il 01/04/20 alle ore 17:53:11