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Titolo:
Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria
Autore:
Hou, SB; Freitas, T; Larsen, RW; Piatibratov, M; Sivozhelezov, V; Yamamoto, A; Meleshkevitch, EA; Zimmer, M; Ordal, GW; Alam, M;
Indirizzi:
Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA Univ Hawaii Honolulu HI USA 96822 Dept Microbiol, Honolulu, HI 96822 USA Univ Hawaii, Dept Chem, Honolulu, HI 96822 USA Univ Hawaii Honolulu HI USA 96822 waii, Dept Chem, Honolulu, HI 96822 USA Univ Florida, Whitney Lab, St Augustine, FL 32080 USA Univ Florida St Augustine FL USA 32080 ey Lab, St Augustine, FL 32080 USA Univ Illinois, Dept Biochem, Urbana, IL 61801 USA Univ Illinois Urbana ILUSA 61801 ois, Dept Biochem, Urbana, IL 61801 USA Marine Bioprod Engn Ctr, Honolulu, HI 96822 USA Marine Bioprod Engn Ctr Honolulu HI USA 96822 Ctr, Honolulu, HI 96822 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 16, volume: 98, anno: 2001,
pagine: 9353 - 9358
SICI:
0027-8424(20010731)98:16<9353:GSACOH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHIZOBIUM-MELILOTI; OXYGEN SENSOR; PAS DOMAINS; PROTEIN; BINDING; AUTOXIDATION; HEMOGLOBIN; SEQUENCES; MYOGLOBIN; REDOX;
Keywords:
proximal histidine; transducer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Alam, M Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA Univ Hawaii Honolulu HI USA 96822 crobiol, Honolulu, HI 96822 USA
Citazione:
S.B. Hou et al., "Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria", P NAS US, 98(16), 2001, pp. 9353-9358

Abstract

The recently discovered prokaryotic signal transducer HemAT, which has been described in both Archaea and Bacteria, mediates aerotactic responses. The N-terminal regions of HemAT from the archaeon Halobacterium salinarum (HemAT-Hs) and from the Gram-positive bacterium Bacillus subtilis (HemAT-Bs) contain a myoglobin-like motif, display characteristic heme-protein absorption spectra, and bind oxygen reversibly. Recombinant HemAT-Hs and HemAT-Bs shorter than 195 and 176 residues, respectively, do not bind heme effectively. Sequence homology comparisons and three-dimensional modeling predict that His-123 is the proximal heme-binding residue in HemAT from both species. The work described here used site-specific mutagenesis and spectroscopy to confirm this prediction, thereby providing direct evidence for a functionaldomain of prokaryotic signal transducers that bind heme in a globin fold. We postulate that this domain is part of a globin-coupled sensor (GCS) motif that exists as a two-domain transducer having no similarity to the PER-ARNT-SIM (PAS)-domain superfamily transducers. Using the GCS motif, we have identified several two-domain sensors in a variety of prokaryotes. We have cloned, expressed, and purified two potential globin-coupled sensors and performed spectral analysis on them. Both bind heme and show myoglobin-like spectra. This observation suggests that the general function of GCS-type transducers is to bind diatomic oxygen and perhaps other gaseous ligands, and to transmit a conformational signal through a linked signaling domain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:39:51