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Titolo:
5-fluorouracil induces autophagic degeneration in rat oral keratinocytes
Autore:
von Bultzingslowen, I; Jontell, M; Hurst, P; Nannmark, U; Kardos, T;
Indirizzi:
Univ Gothenburg, Fac Odontol, Dept Endodontol & Oral Diag, S-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden S-40530 ag, S-40530 Gothenburg, Sweden Univ Otago, Sch Med, Dept Anat & Struct Biol, Dunedin, New Zealand Univ Otago Dunedin New Zealand Anat & Struct Biol, Dunedin, New Zealand Univ Gothenburg, Fac Med, Inst Anat & Cell Biol, S-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden S-40530 ol, S-40530 Gothenburg, Sweden Univ Otago, Sch Dent, Dept Oral Sci & Orthodont, Dunedin, New Zealand UnivOtago Dunedin New Zealand al Sci & Orthodont, Dunedin, New Zealand
Titolo Testata:
ORAL ONCOLOGY
fascicolo: 6, volume: 37, anno: 2001,
pagine: 537 - 544
SICI:
1368-8375(200109)37:6<537:5IADIR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; FLOW-CYTOMETRY; CHEMOTHERAPEUTIC-AGENTS; INTESTINAL EPITHELIA; DNA FRAGMENTATION; CAUTIONARY NOTE; APOPTOSIS; MUCOSITIS; NECROSIS; IDENTIFICATION;
Keywords:
apoptosis; flow cytometry; fluorouracil; in situ nick-end labeling; necrosis; microscopy; electron; mouth mucosa; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: von Bultzingslowen, I Univ Gothenburg, Fac Odontol, Dept Endodontol & OralDiag, Box 450, S-40530 Gothenburg, Sweden Univ Gothenburg Box 450 Gothenburg Sweden S-40530 n
Citazione:
I. von Bultzingslowen et al., "5-fluorouracil induces autophagic degeneration in rat oral keratinocytes", ORAL ONCOL, 37(6), 2001, pp. 537-544

Abstract

In this study, we investigated the effect of 5-fluorouracil (S-FU) on the keratinocytes of oral epithelium. Female Lewis rats were given 5-FU i.v. and were killed 12, 24 or 36 h after injection. The buccal mucosa was dissected. The number of nuclei with DNA strand breaks and the total number of nuclei per volume of the epithelial basal cell layer was estimated using terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling. Epithelial cells were analysed by flow cytometry, transmission electron microscopy and a dye exclusion test. The number of cells with DNA strand breaks increased in S-FU treated rats. Flow cytometry showed a decrease in cell size and an increase in granularity with increasing doses of 5-FU. Dye exclusion gave no indication of degenerate cellmembranes. By transmission electron microscopy, the cells showed evidence of degeneration, shrinkage and loss of cell-to-cell contact. Vacuolation was extensive and, in contrast to apoptotic cells, nuclear chromatin condensation seemed to occur centrally in the nuclei. The results show that 5-FU treatment in vivo induces alterations in rat oral keratinocytes that are consistent with autophagic degeneration. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:48:24