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Titolo:
In situ staining with DNA-binding fluorescent dye, Hoechst 33258, to detect microorganisms in the epithelial cells of oral leukoplakia
Autore:
Mizuki, H;
Indirizzi:
Oita Med Univ, Dept Oral & Maxillofacial Surg, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 Maxillofacial Surg, Oita 8795593, Japan
Titolo Testata:
ORAL ONCOLOGY
fascicolo: 6, volume: 37, anno: 2001,
pagine: 521 - 526
SICI:
1368-8375(200109)37:6<521:ISSWDF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PAPILLOMAVIRUS DNA; HAIRY LEUKOPLAKIA; MYCOPLASMA CONTAMINATION; LESIONS; CULTURES; HYBRIDIZATION; VIRUSES;
Keywords:
leukoplakia; mouth mucosa; fluorescent dyes; bisbenzimide; Hoechst 33258 (Hoe-33258); microorganisms; etiology; staining; fluoroscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Mizuki, H Oita Med Univ, Dept Oral & Maxillofacial Surg, Hasamamachi, Oita8795593, Japan Oita Med Univ Hasamamachi Oita Japan 8795593 ita 8795593, Japan
Citazione:
H. Mizuki, "In situ staining with DNA-binding fluorescent dye, Hoechst 33258, to detect microorganisms in the epithelial cells of oral leukoplakia", ORAL ONCOL, 37(6), 2001, pp. 521-526

Abstract

This study was performed to investigate the presence of microorganisms in the epithelial cells of leukoplakia. Frozen sections of 20 specimens of leukoplakia were stained with DNA-binding bisbenzimide Hoechst 33258. As a control, 20 specimens of normal oral mucosa and five specimens of normal skin were used. In all preparations of leukoplakia, small granular fluorescing structures were observed within the cytoplasm of the epithelial cells, predominantly within the cytoplasm of prickle cells, although the amount of the granular structures varied between specimens, layers of the epithelium and even areas of the epithelium within a single section. Less granular structures were observed, or none at all, in the cytoplasm of the epithelial cellsof normal mucosa. No structures were observed in the cytoplasm of the epithelium of skin. The results in this study strongly suggest that microorganisms are present in the epithelial cells of oral mucosa, and that they are closely associated with the development of oral leukoplakia. It is postulated that the microorganisms in the epithelial cells could be bacteria, particularly mycoplasmas. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 06:41:29