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Titolo:
Constitutive expression of ectopic c-Myc delays glucocorticoid-evoked apoptosis of human leukemic CEM-C7 cells
Autore:
Medh, RD; Wang, AX; Zhou, F; Thompson, EB;
Indirizzi:
Univ Texas, Med Branch, Dept Human Biol Chem & Genet, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 l Chem & Genet, Galveston, TX 77555 USA
Titolo Testata:
ONCOGENE
fascicolo: 34, volume: 20, anno: 2001,
pagine: 4629 - 4639
SICI:
0950-9232(20010802)20:34<4629:CEOECD>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; MEDIATED APOPTOSIS; GENE-TRANSCRIPTION; INDUCED RELEASE; BINDING DOMAIN; GROWTH ARREST; OKADAIC ACID; E-BOX; PROTEIN; CYCLE;
Keywords:
cell cycle; caspase 3; TUNEL; Max; p53; p27;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Medh, RD Univ Texas, Med Branch, Dept Human Biol Chem & Genet, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 Genet, Galveston, TX 77555 USA
Citazione:
R.D. Medh et al., "Constitutive expression of ectopic c-Myc delays glucocorticoid-evoked apoptosis of human leukemic CEM-C7 cells", ONCOGENE, 20(34), 2001, pp. 4629-4639

Abstract

Sensitivity to glueocorticoid (GC)-evoked apoptosis in lymphoid cell linescorrelates closely with GC-mediated suppression of c-Myc expression. To establish a functional role for c-Myc in GC-mediated apoptosis, we have stably expressed MycER (TM), the human c-Myc protein fused to the modified ligand-binding domain of the murine estrogen receptor a, in GC-sensitive CEM-C7-14 cells. In CEM-C7-14 cells, MycER (TM) constitutively imparts c-Myc functions. Cells expressing MycERT (TM) (C7-MycER (TM)) exhibited a marked reduction in cell death after 72 h in 100 nm dexamethasone (Dex), with 10-20-fold more viable cells when compared to the parental CEM-C7-14 clone. General GC responsiveness was not compromised, as evidenced by Dex-mediated suppression of endogenous c-Mye and cyclin D3, and induction of c-Jun and the glucocorticoid receptor. MycER (TM) also blunted Dex-mediated upregulation of p271(kipI) and suppression of the Myc target p53. In comparison to parental CEM-C7-14 cells, Dex-evoked DNA strand breaks were negligible and caspase activation was delayed, but the extent of GI cell cycle arrest was similar in C7-MycER (TM) cells. Myc-ER (TM) did not result in permanent, complete resistance to GC however, and the GC-treated cells eventually died, indicative of redundant or interactive mechanisms in the GC-evoked lytic response oflymphoid cells. Our results emphasize the importance of c-Myc suppression in GC-evoked apoptosis of CEM-C7-14 cells.

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Documento generato il 29/03/20 alle ore 15:43:57