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Titolo:
Postnatal differentiation of unipolar brush cells and mossy fiber-unipolarbrush cell synapses in rat cerebellum
Autore:
Morin, F; Dino, MR; Mugnaini, E;
Indirizzi:
Northwestern Univ, Inst Neurosci, Chicago, IL 60611 USA Northwestern UnivChicago IL USA 60611 st Neurosci, Chicago, IL 60611 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 104, anno: 2001,
pagine: 1127 - 1139
SICI:
0306-4522(2001)104:4<1127:PDOUBC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
METABOTROPIC GLUTAMATE RECEPTORS; SUBUNIT MESSENGER-RNAS; IMMUNOHISTOCHEMICAL LOCALIZATION; COCHLEAR NUCLEUS; NMDA RECEPTORS; IMMUNOCYTOCHEMICAL LOCALIZATION; CALRETININ-IMMUNOREACTIVITY; SYNAPTIC CURRENTS; GRANULE CELLS; CORTEX;
Keywords:
calretinin; development; electron microscopy; light microscopy; glutamate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Mugnaini, E Northwestern Univ, Inst Neurosci, 5-474 Searle Bldg,320 E Super St, Chicago, IL 60611 USA Northwestern Univ 5-474 Searle Bldg,320 E SuperSt Chicago IL USA 60611
Citazione:
F. Morin et al., "Postnatal differentiation of unipolar brush cells and mossy fiber-unipolarbrush cell synapses in rat cerebellum", NEUROSCIENC, 104(4), 2001, pp. 1127-1139

Abstract

The unipolar brush cells are excitatory. cerebellar granular layer interneurons that receive mossy fiber input on their dendritic brushes in the formof a giant glutamatergic synapse. We investigated the postnatal development of the brush of the unipolar brush cell in lobules IX and X by light microscopy and defined the maturation of mossy fiber-unipolar brush cell synapses and mossy fiber-granule cell synapses by electron microscopy using calretinin immunocytochemistry to identify unipolar brush cells. During the first postnatal week, unipolar brush cells possessed one or two short, brancheddendrites. The brush differentiated primarily during the successive 21 postnatal (P) days, during which it underwent progressive maturation, This developmental process was subdivided into stages 1-4, which were descriptivelytermed protodendritic unipolar brush cell (P2-12), filopodial brush (P12-16). intermediate brush (P16-21). and dendriolar brush (P21-28) stages. Electron microscopic measurements of individual mossy fiber-unipolar brush celland mossy fiber-granule cell synaptic junctions were made at P12. 16, 21, and 28. While the average length of mossy fiber-unipolar brush cell synapses increased during development, that of mossy fiber-granule cell synapses decreased. Comparisons of the lengths of mossy fiber-unipolar brush cell andmossy fiber-granule cell synapses demonstrated that mossy fiber-unipolar brush cell synapses were longer on average than mossy fiber-granule cell synapses for all ages. Frequency distribution histograms also showed that the percentage of mossy fiber-Unipolar brush cell synapses longer than 0.5 mum was lower in the pooled P12-P16 groups than in the pooled P21-P28 groups (8versus 20%). In contrast, mossy fiber-granule cell synapses longer than 0.5 mum were a small minority at P12, 16. and 21. and occurred rarely at P28. The present study indicates that mossy fiber-unipolar brush cell synapses increase in length with the differentiation of the brush dendrioles. while that of mossy fiber-granule cell synapses decrease with differentiation of the granule cell dendritic claws. The finding that mossy fiber-unipolar brush cell synapses were generally longer than mossy, fiber-granule cell synapses may indicate that the properties of the postsynaptic targets play a major role in shaping synaptic appositions within cerebellar glomeruli. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:22:03