Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Quantitative measurements of mouse brain thrombin-like and thrombin inhibition activities
Autore:
Beilin, O; Gurwitz, D; Korczyn, AD; Chapman, J;
Indirizzi:
Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 rmacol, IL-69978 Tel Aviv, Israel Tel Aviv Univ, Sackler Fac Med, Dept Neurol, IL-69978 Tel Aviv, Israel TelAviv Univ Tel Aviv Israel IL-69978 Neurol, IL-69978 Tel Aviv, Israel Tel Aviv Univ, Sackler Fac Med, Dept Human Genet & Mol Med, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 ol Med, IL-69978 Tel Aviv, Israel
Titolo Testata:
NEUROREPORT
fascicolo: 11, volume: 12, anno: 2001,
pagine: 2347 - 2351
SICI:
0959-4965(20010808)12:11<2347:QMOMBT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MESSENGER-RNA; RAT-BRAIN; PROLYL ENDOPEPTIDASE; ALZHEIMERS-DISEASE; ANTITHROMBIN-III; EXPRESSION; INJURY; THROMBOMODULIN; PEPTIDE;
Keywords:
bestatin; coagulation; hirudin; prolyl endopeptidase; protease nexin-1; prothrombin; serpins; thrombin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Chapman, J Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 69978 Tel Aviv, Israel
Citazione:
O. Beilin et al., "Quantitative measurements of mouse brain thrombin-like and thrombin inhibition activities", NEUROREPORT, 12(11), 2001, pp. 2347-2351

Abstract

Thrombin-like enzymatic activity was measured in mouse brain homogenates and slices by cleavage of a peptide substrate, N-p-Tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin. The activity was localized mainly to white matter. However, it was not affected by specific thrombin inhibitors, and was found to represent the sum of at least two enzyme activities, a prolyl endopeptidase and an aminopeptidase. By specifically inhibiting this endogenous activity in combination with exogenously added thrombin, mouse brain tissue was shown to express a capacity of thrombin inhibitory activity equivalent to 0.2 mU thrombin/mg brain tissue. The present study offers a simple and reliable method for measuring total thrombin inhibitory activity in brain. NeuroReport 12:2347-2351 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:59:41