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Titolo:
Amyloid beta peptide 1-40 and the function of rat hippocampal hemicholinium-3 sensitive choline carriers: Effects of a proteolytic degradation in vitro
Autore:
Kristofikova, Z; Tejkalova, H; Klaschka, J;
Indirizzi:
Prague Psychiat Ctr, Prague 18103 8, Bohnice, Czech Republic Prague Psychiat Ctr Prague Bohnice Czech Republic 18103 8 Czech Republic Inst Comp Sci, Prague 18200 8, Czech Republic Inst Comp Sci Prague CzechRepublic 18200 8 ague 18200 8, Czech Republic
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 3, volume: 26, anno: 2001,
pagine: 203 - 212
SICI:
0364-3190(200103)26:3<203:ABP1AT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-AFFINITY; ALZHEIMERS-DISEASE; BINDING-SITES; IN-VITRO; CALCIUM CHANNELS; STEM BROMELAIN; CELL-DEATH; BRAIN; ETHANOL; PROTEIN;
Keywords:
abeta; bromelain; papain; choline uptake; hemicholinium-3; rat hippocampus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kristofikova, Z Prague Psychiat Ctr, Ustavni 91, Prague 18103 8, Bohnice, Czech Republic Prague Psychiat Ctr Ustavni 91 Prague Bohnice Czech Republic18103 8
Citazione:
Z. Kristofikova et al., "Amyloid beta peptide 1-40 and the function of rat hippocampal hemicholinium-3 sensitive choline carriers: Effects of a proteolytic degradation in vitro", NEUROCHEM R, 26(3), 2001, pp. 203-212

Abstract

Effects of amyloid beta peptide 1-40 (Abeta) and of plant cysteine proteases bromelain and papain on the high-affinity uptake of choline (HACU) and the specific binding of [H-3]hemi-cholinium-3 ([H-3]HC-3) have been investigated on hippocampal synaptosomes from young adult male Wistar rats under basal and stimulated conditions (55 mM KCI). Depolarization increased significantly the HACU levels (the changes were predominantly in Vmax) and mildly the [3H]HC-3 binding (the changes especially in K-D). Nonaggregated Abeta at low nM concentrations suppressed the depolarization effects but was ineffective under basal conditions during a short-term incubation. Higher muM concentrations decreased the HACU and binding under basal conditions in a time-dependent manner. The binding changes were firstly associated with alterations in K-D and secondarily were accompanied also by a drop in Bmax. The results suggest that Abeta directly influences high-affinity carriers, inhibits their transport activity and enhances their sensitivity to proteolytic cleavage. Stimulation increases the sensitivity of carriers to the interaction with Abeta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:24:25