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Titolo:
Plant viral genes in DNA idiotypic vaccines activate linked CD4(+) T-cell mediated immunity against B-cell malignancies
Autore:
Savelyeva, N; Munday, R; Spellerberg, MB; Lomonossoff, GP; Stevenson, FK;
Indirizzi:
Southampton Univ Hosp Trust, Tenovus Lab, Mol Immunol Grp, Southampton SO16 6YD, Hants, England Southampton Univ Hosp Trust Southampton Hants England SO16 6YD s, England John Innes Inst, Dept Virus Res, Norwich NR4 7UH, Norfolk, England John Innes Inst Norwich Norfolk England NR4 7UH NR4 7UH, Norfolk, England
Titolo Testata:
NATURE BIOTECHNOLOGY
fascicolo: 8, volume: 19, anno: 2001,
pagine: 760 - 764
SICI:
1087-0156(200108)19:8<760:PVGIDI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOBACCO MOSAIC-VIRUS; DENDRITIC CELLS; IN-VIVO; ANTIGEN PRESENTATION; PROTECTIVE IMMUNITY; FRAGMENT-C; IFN-GAMMA; IMMUNIZATION; LYMPHOMA; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Stevenson, FK Southampton Univ Hosp Trust, Tenovus Lab, Mol Immunol Grp, Southampton SO16 6YD, Hants, England Southampton Univ Hosp Trust Southampton Hants England SO16 6YD
Citazione:
N. Savelyeva et al., "Plant viral genes in DNA idiotypic vaccines activate linked CD4(+) T-cell mediated immunity against B-cell malignancies", NAT BIOTECH, 19(8), 2001, pp. 760-764

Abstract

DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B-cell tumors, encoded by the variable region genes, areclone-specific tumor antigens. When assembled as single-chain Fv (ScFv) alone in a DNA vaccine, immunogenicity is low. Previously, we found that fusion of a sequence from tetanus toxin (fragment C; FrC) promoted anti-idiotypic protection against lymphoma and myeloma. We have now investigated an alternative fusion gene derived from a plant virus, potato virus X coat protein, a primary antigen in humans. When fused to scFv, the self-aggregating protein generates protection against lymphoma and myeloma. In contrast to scFv-FrC, protection against lymphoma is mediated by CD4(+) T cells, as is protection against myeloma. Plant viral proteins offer new opportunities to activate immunity against linked T-cell epitopes to attack cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:36:32