Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cell-free translation reconstituted with purified components
Autore:
Shimizu, Y; Inoue, A; Tomari, Y; Suzuki, T; Yokogawa, T; Nishikawa, K; Ueda, T;
Indirizzi:
Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Kashiwa, Chiba 2778562,Japan Univ Tokyo Kashiwa Chiba Japan 2778562 hnol, Kashiwa, Chiba 2778562,Japan Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan Univ Tokyo Kashiwa Chiba Japan 2778562 sci, Kashiwa, Chiba 2778562, Japan Gifu Univ, Fac Engn, Dept Biomol Sci, Gifu 5011193, Japan Gifu Univ GifuJapan 5011193 Engn, Dept Biomol Sci, Gifu 5011193, Japan
Titolo Testata:
NATURE BIOTECHNOLOGY
fascicolo: 8, volume: 19, anno: 2001,
pagine: 751 - 755
SICI:
1087-0156(200108)19:8<751:CTRWPC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNNATURAL AMINO-ACIDS; ESCHERICHIA-COLI; MESSENGER-RNA; FREE SYSTEM; PROTEIN; INVITRO; GENE; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Ueda, T Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bldg FSB401,5-1-5 Kasiwanoha, Kashiwa, Chiba 2778562, Japan Univ Tokyo Bldg FSB401,5-1-5 Kasiwanoha Kashiwa Chiba Japan 2778562
Citazione:
Y. Shimizu et al., "Cell-free translation reconstituted with purified components", NAT BIOTECH, 19(8), 2001, pp. 751-755

Abstract

We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able toproduce protein at a rate of about 160 mug/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 04:33:49