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Titolo:
Musk ketone enhances benzo(a)pyrene induced mutagenicity in human derived Hep G2 cells
Autore:
Mersch-Sundermann, V; Schneider, H; Freywald, C; Jenter, C; Parzefall, W; Knasmuller, S;
Indirizzi:
Univ Trier, FB 6, Dept Toxicol & Ecotoxicol, D-54286 Trier, Germany Univ Trier Trier Germany D-54286 ol & Ecotoxicol, D-54286 Trier, Germany Univ Heidelberg, Inst Microbiol & Hyg, Fac Clin Med Mannheim, D-68135 Mannheim, Germany Univ Heidelberg Mannheim Germany D-68135 heim, D-68135 Mannheim, Germany Univ Vienna, Inst Canc Res, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Inst Canc Res, A-1090 Vienna, Austria
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 495, anno: 2001,
pagine: 89 - 96
SICI:
1383-5718(20010822)495:1-2<89:MKEBIM>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEPATOMA-CELL; POLYCYCLIC AROMATIC-HYDROCARBONS; DRUG-METABOLIZING-ENZYMES; NITRO MUSKS; SOS-CHROMOTEST; TAMA RIVER; XYLENE; GENOTOXICITY; CYTOCHROME-P-450; LINE;
Keywords:
Hep G2/MN assay; musk ketone; benzo(a)pyrene; comutagenic;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Mersch-Sundermann, V Univ Trier, FB 6, Dept Toxicol & Ecotoxicol, D-54286 Trier, Germany Univ Trier Trier Germany D-54286 286 Trier, Germany
Citazione:
V. Mersch-Sundermann et al., "Musk ketone enhances benzo(a)pyrene induced mutagenicity in human derived Hep G2 cells", MUT RES-GTE, 495(1-2), 2001, pp. 89-96

Abstract

Musk ketone is a widely used artificial fragrance which has been identified in human fatty tissue and milk. The mutagenic and comutagenic effects of this compound were studied in micronucleus tests with a human derived hepatoma cell line (Hep G2). Exposure of the cells to MK alone in the range between 5 and 5000 ng/ml did not cause induction of MN. When the cells were treated simultaneously with MK (5-5000 ng/ml) and 0.2 mug/ml benzo(a)pyrene, no synergistic effects were detected; benzo(a)pyrene (B(a)P) itself caused an 1.5-fold increase of MN over the spontaneous background frequency (60 versus 39 MN/1000 binucleated cells). In a third experimental series, the cells were pretreated with MK for 28 h and subsequently exposed to 0.2 mug/ml B(a)P. In this case, a pronounced comutagenic effect was observed: The LOAELfor MK was 0.05 mug/ml. With higher doses (0.5, 1.0 and 5.0 mug MK/ml), a significant increase of B(a)P induced MN frequencies was measured, the induction rates being 50, 66, and 88%, respectively. Additional measurements of7-ethoxyresorufin deethylase indicated that MK induces cytochrome P450 isoenzymes (1A1) which play a key role in the activation of B(a)P. The resultsof the present study show that MK amplifies the genotoxic effects of B(a)Pin human derived cells and indicate that exposure of humans to MK might increase their susceptibility to the health hazards of B(a)P and other polycyclic aromatic hydrocarbons. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 06/04/20 alle ore 02:53:40